2型糖尿病膀胱大鼠模型的制作及尿流动力学变化

目的 探讨2型糖尿病膀胱大鼠模型的制作方法及其尿流动力学变化情况.方法 将40只雌性大鼠体 (200250) g分为2组:A组为对照组 (22只) , B组为实验组 (18只) .实验组经高糖高脂喂养四周后腹腔内注射链脲佐菌素 (STZ) (30 mg/kg一次性腹腔注射) 诱导建立大鼠2型糖尿病膀胱模型;对照组常规饲养4周后腹腔注射相同剂量枸橼酸缓冲液.2组均采用尾静脉测定大鼠血糖, 每周1次, 连续4周.12周时2组大鼠进行膀胱容量、膀胱压及漏尿点压测定, 连续记录3次.结果 与对照组相比较, 实验组血糖明显升高, STZ建模成功率100%;尿动力学结果显示实验组充盈期膀胱容量明显高于对照组, 膀胱压和漏尿点明显低于对照组.结论 STZ用于建立糖尿病大鼠膀胱模型成功率高, 其膀胱尿道功能发生显著改变.

Preparation of Rat Model of Diabetic Cystopathy (DCP) and the Changes of Urodynamics of Rats with DCP

Objective To explore the modeling of diabetic cystopathy (DCP) and the changes of urodynamics in rats with DCP.Methods 40 female rats (200-250 g) were divided into two groups: group A, the controlled group (22 rats) and group B (18 rats) , the experimental group.The model of DCP (experimental group) was established with the intraperitoneal injection of streptozotocin (STZ) after 4 weeks feeding of high-sugar high-fat.Group A was injected citrate buffer intraperitoneally (the same dose of STZ) after 4 weeks normal feeding.Both groups were tested the blood sugar through the tail vein, 1 time a week, four times altogether.Twelve weeks later, the bladder capacity, bladder pressure and leakage point pressure were tested with urodynamic instument.The results were tested 3 times.Results The blood sugar of group B was higher obviously than group A.And the successful modeling rate of DCP with STZ was 100%.Compared wth group A, the filling bladder capacity was higher significantly, the bladder pressure and leakage point pressure were lower significantly in group B.Conclusion The successful modeling rate of DCP with STZ is high and the urethra and bladder function changes are significant in DCP rats.