胃癌不同组织学分型与微血管密度及相关因子的关系*

目的：探讨微血管密度（microvascular density，MVD），基质金属蛋白酶- 2（matrix metalloproteinase-2，MMP-2）、基质金属蛋白酶- 9（matrix metalloproteinase-9，MMP-9）、血管内皮生长因子（vascular endothelial growthfactor，VEGF）、血管内皮生长因子受体1（vascular endothelial growth factor receptor 1，VEGFR1）、血管内皮生长因子受体2（vascularendothelialgrowthfactorreceptor 2，VEGFR2）、p53蛋白与胃癌Lauren 分型及WHO分型的关系。方法：收集89例胃癌患者的临床数据并将其按照Lauren 和WHO标准进行分型。应用单克隆抗体CD34/PAS 双重染色评价89例胃癌组织中的微血管密度，免疫组织化学法检测标本中MMP-2、MMP-9、VEGF、VEGFR1、VEGFR2 和p53蛋白的表达。结果：在胃癌中，MVD与Lauren 分型和WHO分型均无相关性（P > 0.05）。 Lauren 分型与MMP-9、VEGFR1 和p53有相关性（P < 0.05），与MMP-2、VEGF和VEGFR2 无相关性（P > 0.05）。 WHO分型与各肿瘤相关因子均无相关性（P > 0.05）。 Lauren 分型和WHO分型是胃癌患者独立的预后因素（P < 0.05）。 结论：对肿瘤相关因子、血管生成与胃癌发生、发展过程的研究，可能为不同组织学分型的胃癌患者提供新的治疗方法。

Relationship of different histological classifications of gastric cancer with microvessel density and related factors

Objective:To investigate the correlations of Lauren classification and world health organization (WHO) classification of gastric cancer (GC) with microvascular density (MVD), matrix metalloproteinase- 2 (MMP- 2), matrix metalloproteinase- 9 (MMP- 9), vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor1 (VEGFR 1), vascular endothelial growth factor receptor 2 (VEGFR 2), and p53 . Methods:The clinical data of 89 patients with GC were collected. The collected specimens were categorized on the basis of Lauren classification and WHO classification. CD34 /periodic acid-Schiff (PAS) double staining was performed to validate MVD. Immunohistochemistry was conducted to investigate the expression levels of MMP- 2, MMP-9, VEGF, VEGFR 1, VEGFR 2, and p 53 . Results: MVD was not correlated with Lauren classification or WHO classification (P>0.05 ). Lauren typing was associated with the expression levels of MMP- 9, VEGFR 1, and p 53 (P<0.05 ). WHO classification was not related to any of the factors (P>0.05 ). Cox proportional hazards model revealed that Lauren classification and WHO classification were the prognostic factors of overall survival (P<0.05 ). Conclusion: This research on tumor related factors, angiogenesis, and different classifications of GC may provide new methods to treat this disease.