miR-21及其靶蛋白RASA1在寻常型银屑病患者皮肤组织中的表达及意义

目的 探讨miR-21在寻常型银屑病患者皮损组织、皮损旁组织以及正常健康人群皮肤组织中的表达水平,同时研究其下游靶蛋白RASA1在寻常型银屑病患者皮损组织和正常人群皮肤组织中的表达水平及意义。 方法 采用实时荧光定量PCR法检测miR-21在12例寻常型银屑病患者皮损组织、皮损旁组织以及15例正常人群皮肤组织中的表达水平,差别倍数用2-ΔΔCt值表示。利用免疫组织化学Envision法检测45例寻常型银屑病患者与20例正常人群皮肤组织中RASA1蛋白的表达情况,RASA1阳性显色主要为细胞质显示棕黄色或棕褐色颗粒。 结果 经过实时荧光定量PCR检测后分析显示:寻常型银屑病患者皮损组织中miR-21的表达水平显著高于正常皮肤组织,差异具有统计学意义(P<0.01)。免疫组化结果表明:RASA1蛋白在正常皮肤组织中的阳性表达率为60.00%,显著高于寻常型银屑病患者皮损中的阳性表达率(10.00%),差异具有统计学意义(χ2=14.009,P<0.001)。 结论 miR-21在寻常型银屑病皮损组织中的表达高于正常组织,其靶蛋白RASA1在寻常型银屑病患者皮肤组织中的阳性表达显著低于正常皮肤组织;可能是由于miR-21的差异表达调控RASA1基因,导致RASA1蛋白表达受到抑制,从而参与指向寻常型银屑病的发病过程。 

Expression and significance of miR-21 and its target protein RASA1 in the skin of patients with psoriasis vulgaris

Objective To investigate the expression of miR-21 and its target protein RASA1 in skin of patients with psoriasis vulgaris and its significance in the development of psoriasis vulgaris. Methods The expressions of miR-21 were studied with real-time fluorescent quantitative PCR in the 12 psoriatic vulgaris lesions and beside-lesional tissue and 15 normal skin, differential multiple representation with 2-ΔΔCt.The expressions of RASA1 were studied with immunohistochemical in the 45 psoriatic and 20 normal skin.The positive mainly as brown or tan particles in cytoplasm. Results With real-time fluorescent quantitative PCR, the expression level of miR-21 was higher in lesions of psoriasis vulgaris than that in the beside-lesional tissue and normal skin tissue, and differences was statistical significance (P < 0.01).The positive expression of RASA1 in skin lesions of patients with psoriasis population was 10.00%, it's lower than 60.00% the rate of positive expression in the normal group.The data were statistically significant (χ2=14.009, P < 0.001). Conclusion The expression of miR-21 is higher in lesions of psoriasis vulgaris than that in the normal skin tissue, but the positive expression of its target protein RASA1 is lower in lesions of psoriasis vulgaris.Differential expression of miR-21 may be regulation of RASA1 gene, which can inhibit the expression of RASA1 protein.These may lead to psoriasis vulgaris.