| Let-7在正常乳腺组织和乳腺癌组织中的表达 |
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| 作者姓名： | 喻箴 |
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| 作者单位： | (1)昆明医科大学公共卫生学院, 云南 昆明 650500;2)昆明医科大学第三附属医院,云南 昆明 650118 ;3)成都军区昆明总医院普通外科,云南 昆明 650031) |
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| 基金项目： | ［基金项目］云南省自然科学基金资助项目（2009CD081）；云南省教育厅科研基金资助项目（2011Y176） |
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| 摘 要： | ［摘要］目的 研究Let-7基因在乳腺癌中的表达特征．方法 随机抽取28例乳腺癌患者收集其癌组织和癌旁正常组织，利用TRIzol提取总RNA，实时荧光定量PCR检测基因表达量．结果 Let-7前体在癌组织中的相对表达量（9.65±2.31），低于癌旁正常组织（10.0±52.81），P＝0.048，而Let-7基因则无显著变化．前体Let-7与最长行经（日）之间有依存关系（b＝0.875，P＝0.020）；初潮年龄与前体Let-7的表达在癌组织中的呈负相关（r＝-0.484，P＝0.049）， 在正常组织中呈正相关（r＝0.502，P＝0.048）．结论 Let-7前体在癌组织中的表达低于正常组织， 最长行经天数越长则Let-7表达越高．
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| 关 键 词： | [关键词]乳腺癌 Let-7 基因 |
Expressions of Let-7 in Normal Breast Tissue and Breast Cancer Tissue |
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| Institution: | (1)School of Public Health,Kunming Medical University,Kunming Yunnan 650500;2)Dept. of Cardio-Thoracic Surgery,The Third Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650118;3)Dept. of General Surgery,Kunming General Hospital of Chengdu Military Region,Kunming Yunnan 650031, China) |
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| Abstract: | ［Abstract］Objective To study the expression characteristics of Let-7 genes in breast cancer. Methods Twenty-eight patients with breast cancer were randomly selected，and their cancer tissue and adjacent normal tissue were collected. TRIzol was used to extract the total RNA and real-time quantitative PCR was used to detect the relative gene expression levels. Results The expression of precursor Let-7 in cancer tissue was（9.65±2.31），which was lower than that in normal tissue（10.05±2.81 ），P = 0.048. Precursor Let-7 had dependence relationship with the long menstruation（b = 0.816，P = 0.029）. The menarche age showed a positive correlation with precursor Let-7 in normal tissue（r = 0.502，P = 0.048）and a negative correlation in cancer tissue（r = -0.484，P = 0.049）. Conclusions The expression of precursor Let-7 in cancer tissue is lower than that in normal tissue. The period of menstruation is a protective factor to breast cancer. |
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