Aurora-A mt-P53和c-myc在大肠癌中的表达及意义

  目的  研究中心体相关激酶Aurora-A、突变型P53(mt-P53)和c-myc在大肠癌中的表达规律, 探讨其相互关系及在肿瘤发生发展中的作用。  方法  应用组织芯片和免疫组织化学技术(SP法)检测Aurora-A、mt-P53和c-myc在130例大肠癌、癌旁组织和正常大肠组织中的表达, 并结合临床病理学参数进行综合分析。  结果  Aurora-A在正常大肠组织、癌旁组织和大肠癌中的阳性表达率分别为0, 35%, 69%。mt-P53分别为0, 20%, 57%。c-myc分别为0, 37%, 76%。与正常大肠组织和癌旁相比, 癌组织中Aurora-A、mt-P53和c-myc的表达明显升高(P < 0.01);三者的表达与肿瘤的浸润程度有关(P < 0.05), mt-P53和c-myc还与Dukes'分期和淋巴结转移关系密切(P < 0.05)。在大肠癌中Aurora-A的阳性表达与mt-P53、c-myc的阳性表达呈显著正相关(分别为r=0.362, P < 0.01;r=0.487, P < 0.01), mt-P53和c-myc之间也存在显著正相关(r=0.242, P < 0.01)。  结论  Aurora-A和c-myc蛋白的过表达与P53的突变在大肠癌的发生、侵袭和转移中发挥着重要作用。综合分析Aurora-A、mt-P53和c-myc蛋白的表达对大肠癌的早期诊断及预后判断具有重要价值。 

Expression of centrosome-associated kinase Aurora-A,mt-P53, and c-myc in colorectal cancer and its significance

  Objective  This study aimed to investigate the expression levels of centrosome-associated kinase Aurora-A, mutant type P53(mt-P53), and c-myc in colorectal cancer.This study was also conducted to investigate the mutual relationship and functions of these factors in tumorigenesis and tumor progression.  Methods  We examined the pathological specimens obtained from colorectal cancer, pericancerous tissues, and normal colorectal tissues by tissue microarray technique and immunohistochemistry (SP method) to determine the expression levels of Aurora-A, mt-P53, and c-myc proteins.The clinicopathological parameters were then analyzed.  Results  The positive rates of Aurora-A expression in normal colorectal tissues, pericancerous tissues, and colorectal cancer were 0%, 35%, and 69% respectively; by comparison, the positive rates of mt-P53 were 0%, 20%, and 57%, respectively.For c-myc, the positive rates were 0, 37%, and 76%, respectively.The expression levels of Aurora-A, mt-P53, and c-myc were significantly higher in tumor tissues than in normal colorectal tissues and pericancerous tissues (P < 0.01).Aurora-A overexpression was related to the depth of invasion (P < 0.05).Mt-P53 and c-myc overexpression was related to the depth of invasion, lymph node metastasis, and Dukes'classification (P < 0.05).A strong positive correlation was observed between the expressions of Aurora-A, mt-P53, and c-myc in colorectal cancer (r= 0.362, P < 0.01;r=0.487, P < 0.01).A strong positive correlation was also observed between the expressions of mt-P53 and c-myc in colorectal cancer (r=0.242, P < 0.01).  Conclusion  The overexpression of Aurora-A and c-myc and the mutation of P53 were important in tumorigenesis, tumor invasion, and metastasis of colorectal cancer.Thus, the co-detection of Aurora-A, mt-P53, and c-myc may be useful for the early diagnosis and prognosis of colorectal cancer.