哮喘患儿外周血淋巴细胞亚群和CD4+ T细胞亚群的变化情况

目的 小儿哮喘的发生发展受免疫系统的影响,本文探讨哮喘患儿外周血淋巴细胞亚群和CD4+T细胞亚群的变化情况,探讨其临床意义。 方法 选择2013年1月-2015年12月哮喘患儿80例作为哮喘组,健康体检儿童80例作为对照组。测定外周血淋巴细胞亚群和CD4+T细胞亚群含量。 结果 哮喘组CD3+、CD4+、CD19+、CD4+/CD8+[(62.13±11.24)%、(32.97±6.57)%、(24.35±11.21)%、(1.66±0.31)%]高于对照组[(71.16±12.64)%、(37.68±7.12)%、(13.87±8.46)%、(1.43±0.28)%,P<0.05],哮喘组CD8+[(19.87±4.21)%]低于对照组[(26.37±4.65)%。P<0.05]。哮喘组Th2、Th17、Treg、Th17/Treg[(4.42±2.14)%、(1.71±0.97)%、(7.65±1.35)%、(0.22±0.05)%]均高于对照组[(1.96±1.25)%、(0.89±0.42)%、(5.91±1.28)%、(0.15±0.03)%,P<0.05],哮喘组Th1/Th2[(1.31±1.32)]低于对照组[(2.70±1.47),P<0.05],2组Th1比较差异无统计学意义(P>0.05)。哮喘组淋巴细胞[(36.57±13.24)%]低于对照组[(59.64±15.47)%,P<0.05],哮喘组单核细胞[(12.17±4.21)%]高于对照组[(3.68±1.74)%,P<0.05],2组白细胞和中性粒细胞比较差异无统计学意义(P>0.05)。淋巴细胞与Th1、Th2、Th17呈负相关(P<0.05),单核细胞与CD3+、CD4+、CD19+呈负相关(P<0.05),与CD8+呈正相关(P<0.05)。 结论 哮喘患儿存在淋巴细胞亚群和CD4+T细胞亚群异常,外周血淋巴细胞亚群和CD4+T细胞亚群含量可用于哮喘患儿免疫功能判断。 

Changes of peripheral blood lymphocyte subsets and CD4+T cell subsets in children with asthma

Objective The development of asthma in children is affected by the immune system,the aim of this paper is to investigate the changes of peripheral blood lymphocyte subsets and CD4+T cell subsets and it clinical significance in children with asthma. Methods Eighty children with asthma (asthma group) and 80 healthy children (control group) in our hospital from January,2013 to December,2015 were enrolled into this study.The peripheral blood lymphocyte subsets and CD4+T cell subsets were measured. Results The levels of CD3+,CD4+,CD19+,CD4+/CD8+in asthma group were (62.13±11.24)%,(32.97±6.57)%,(24.35±11.21)% and (1.66±0.31)%,respectively,which were significantly higher than those in control group[(71.16±12.64)%,(37.68±7.12)%,(13.87±8.46)%,(1.43±0.28)%,P<0.05].The level of CD8+(19.87%±4.21%) in asthma group was lower than that in control group (26.37±4.65%),P<0.05.The levels of Th2,Th17,Treg,Th17/Treg in the asthma group were (4.42±2.14)%,(1.71±0.97)%,(7.65±1.35)% and (0.22±0.05)%,respectively,which were higher than those in the control group[(1.96±1.25)%,(0.89±0.42)%,(5.91±1.28)%,(0.15±0.03)%,P<0.05].The Th1/Th2(1.31±1.32%) in the asthma group was lower than that in the control group (2.70±1.47%),P<0.05.There was no significant difference in Th1 between the two groups (P>0.05).The lymphocytes in the asthma group (36.57±13.24%) was significantly lower than that in the control group (59.64%±15.47%),P<0.05,the monocytes in the asthma group (12.17±4.21%) was higher than the control group (3.68%±1.74%),P<0.05.There was no significant difference in white blood cells and neutrophils between the two groups (P>0.05).The lymphocytes were negatively correlated with Th1,Th2 and Th17(P<0.05).The monocytes were negatively correlated with CD3+,CD4+ and CD19+(P<0.05),and was positively correlated with CD8+(P<0.05). Conclusion The lymphocyte subsets and CD4+T cell subsets in children with asthma are abnormal.Peripheral blood lymphocyte subsets and CD4+T cell subsets can be used to determine the immune function of children with asthma.