枕大池注入利多卡因对兔SAH后基底动脉Rho/ROCK信号传导的影响

目的 探讨枕大池注入利多卡因对兔蛛网膜下腔出血（SAH）后基底动脉血管Rho/Rho相关激酶（ROCK）信号传导的影响。 方法 24只新西兰大白兔随机分为3组，每组8只，分别为假手术（sham）组、蛛网膜下腔出血（SAH）组、枕大池利多卡因（CD）组。SAH组和CD组动物经枕大池注入非抗凝的自体动脉血（1 mL/kg）复制SAH模型，sham组经枕大池注入37℃的生理盐水（1 mL/kg）；30 min后，CD组经枕大池注入0.3 mL 2%利多卡因，SAH组和sham组注入等量生理盐水。72 h后测定摄食量和神经功能损害分级，实时荧光定量PCR（qRT-PCR）和Western blot检测基底动脉中Rho相关激酶2（ROCK2）、肌球蛋白轻链（MLC）及钙调蛋白（CaM）蛋的基因和蛋白表达。 结果 与sham组比较，SAH组和CD组家兔摄食量减少，且出现不同程度的神经功能损害，ROCK2在基底动脉中的mRNA及蛋白表达量增高，MLC和CaM的mRNA和蛋白表达减少（P<0.05）；与SAH组比较，CD组家兔摄食量及神经功能损害差异无统计学意义（P>0.05），ROCK2在基底动脉中的mRNA及蛋白表达量减少，MLC和CaM的mRNA和蛋白表达增高（P<0.05）。 结论 枕大池注入利多卡因能够抑制兔SAH后基底动脉血管Rho/ROCK信号传导，减轻SAH后基底动脉平滑肌的收缩。

Effects of Lidocaine on the Rho/ROCK Signal Transduction of the Posterior Basilar Artery in Rabbit SAH

Objective To observe the expression of Rho/Rho-associated kinase (ROCK) signaling pathway in the basilar artery and the effect of lidocaine on this signaling pathway after subarachnoid hemorrhage (SAH) in rabbits. Methods 24 New Zealand white rabbits were randomly divided into sham operation (sham) group, SAH group, and occipital cisterna lidocaine (CD) group. There were 8 rabbits in each group. Intracisternal injection of non-anticoagulant autologous arterial blood (1 mL/kg) were applied to SAH group and CD group animals to establish SAH model, sham normal saline group was injected with 37℃ physiological saline (1 mL/kg); after 30 min, CD group was injected with 0.3 mL 2% lidocaine cisterna, SAH group and sham group were injected with saline. After 72 h, food intake and neurologic function damage were measured. The expressions of Rho associated kinase 2 (ROCK2) and myosin light chain (MLC) and calmodulin (CaM) protein in the basilar artery were measured by Western blot. The ROCK2 and MLC and CaM gene expressions were measured by using real-time quantitative PCR. Results Compared with sham group, reduced food intake, various degrees of neurological impairment, increased ROCK2 mRNA and protein expressions in basal artery, and decreased MLC and CaM expressions were observed in SAH group and CD group (P<0.05). Compared with the SAH group, there was no statistically significant difference in diet intake and neurological damage in the CD group (P>0.05); the mRNA and protein expressions of ROCK2 in the basilar artery decreased, and the expressions of MLC and CaM increased (P<0.05). Conclusion Intracisternal injection of lidocaine may inhibit the transmission of Rho/ROCK signal in the basilar artery and reduce the basilar artery smooth muscle contraction after SAH.