分泌性中耳炎患者的血清及中耳积液内炎性因子和转化生长因子水平检测及其临床价值分析

目的 探讨分泌性中耳炎(secretory otitis media,SOM)患者血清及耳积液内炎性因子和转化因子水平变化,并分析指标水平与SOM的相关性。 方法 选取2015年10月-2017年1月浙江省立同德医院收治的SOM患者作为观察对象,分为急性组、亚急性组和慢性组,每组各抽取患者30例,同时选取30例健康体检者作为对照组;比较各组患者血清、耳积液内的炎性因子和转化因子水平,并分析各指标与SOM的相关性,其中多组间指标水平比较采用单因素方差分析,组间两两比较采用t检验,相关性分析采用Spearman分析。 结果 SOM患者与对照组血清及SOM患者中耳积液内的炎性因子与转化因子水平比较差异均有统计学意义(均P<0.05);与对照组相比,SOM患者血清TNF-α、IL-5、IL-8、TGF-β1和TGF-β2水平均显著升高,差异均有统计学意义(均P<0.05);与急性组相比,亚急性组及慢性组的血清及中耳积液内的TNF-α、IL-5、IL-8、TGF-β1和TGF-β2水平均显著升高,且慢性组各指标水平显著高于亚急性组,差异均有统计学意义(均P<0.05);相关性分析结果表明,SOM患者血清及中耳积液内的TNF-α、IL-5、IL-8、TGF-β1和TGF-β2水平与SOM病情严重程度呈正相关(均P<0.05)。 结论 SOM患者血清炎性因子及转化生长因子水平异常,血清及耳积液内炎性因子、转化生长因子水平与SOM的病情严重程度关系较为密切。 

Clinical value analysis and detection of inflammatory factors and transforming growth factor levels of serum and middle ear effusion in patients with secretory otitis media

Objective To investigate the levels of inflammatory and transforming factors in serum and auricular fluid in patients with secretory otitis media (SOM) and to analyze the correlation between the level of the index and the SOM. Methods Patients with SOM in our hospital from October, 2015 to January, 2017 were selected as the observation objects. They were divided into acute group, subacute group and chronic group, each group of patients with 30 cases, at the same time 30 healthy persons were selected as control group. The levels of inflammatory factors and transforming factors in the serum and ear fluid were compared between the groups, and the correlation between the indexes and SOM was analyzed. Comparison of multi-group index level was used a single factor analysis of variance levels between the two groups were compared with t test and the correlation analysis was analyzed by Spearman. Results The levels of inflammatory and transforming factors in the serum and middle ear effusion between the SOM patients and the control group were statistically significant (all P<0.05). Compared with the control group the levels of TNF-α, IL-5, IL-8, TGF-β1 and TGF-β2 were significantly increased in SOM patients the difference was statistically significant (all P<0.05). Compared with the acute group levels of TNF-α, IL-5, IL-8, TGF-β1 and TGF-β2 in serum and middle ear effusion of subacute group and chronic group were significantly increased, and the indexes of chronic group were significantly higher than those in subacute group, the difference was statistically significant (all P<0.05). The correlation analysis showed that levels of TNF-α, IL-5, IL-8, TGF-β1 and TGF-β2 levels in serum and middle ear effusion were positively correlated with SOM (all P<0.05). Conclusion Levels of serum inflammatory factors and transforming growth factors in patients with SOM are abnormal, and the inflammatory factors and transforming growth factor levels in serum and ear effusion are closely related to the severity of SOM.