转移抑素抑制人鼻咽癌高转移能力细胞株SUNE-1-5-8F转移侵袭能力的研究

目的 研究并探讨转移抑素(metastin)对人鼻咽癌高转移能力细胞株SUNE-1-5-8F转移侵袭能力的影响。 方法 ①采用Transwell体外侵袭趋化实验技术，在不同浓度(102 nM、103 nM、104 nM)转移抑素干预前后计数转移及侵袭细胞数，探究其对SUNE-1-5-8F细胞转移能力和侵袭能力的影响。②采用BALB/c-nu裸鼠肺转移模型，40只裸鼠随机平均分为转移抑素组及对照组，2组均右侧腋窝下注射2×106/ml SUNE-1-5-8F细胞悬液，其中转移抑素组于第2天按1.17 mg/kg腹腔注射转移抑素，研究其对裸鼠移植瘤的抑瘤率、转移抑制率和生命延长率的影响；采用免疫组化法研究肺转移灶kisspeptin指标表达情况。 结果 ①Transwell实验中转移抑素对于SUNE-1-5-8F细胞的转移能力及侵袭能力均具有抑制作用(P<0.05)，且其抑制能力与转移抑素浓度呈正相关(P<0.05)。②裸鼠实验中转移抑素对抑瘤率无明显影响(P>0.05)，但可以降低肺转移数，提升转移抑制率(P<0.05)，且进一步提高了小鼠的生命延长率(P<0.05)。免疫组化示转移抑素组kisspeptin较对照组高表达。 结论 ①Transwel实验中转移抑素能够抑制人鼻咽癌高转移能力细胞株SUNE-1-5-8F的转移侵袭能力，且转移抑素浓度与其抑制能力呈正相关。②裸鼠实验中转移抑素能够降低肺转移率，延长裸鼠生命，但与腋下移植瘤的增殖无显著相关性。 

A study of metastin on inhibiting the capacity of metastasis and invasion in highly metastatic nasopharyngeal carcinoma SUNE-1-5-8F cells

Objective To investigate and discuss the effect of metastin on inhibiting the capacity of metastasis and invasion in highly metastatic nasopharyngeal carcinoma SUNE-1-5-8F cells. Methods ①The SUNE-1-5-8F cells' were treated with different concentration of metastin(102 nM,103 nM,104 nM).Contrast the cells before and after treatment.Finally,the capacity of metastasis and invasion were tested by transwell assay.②Forty female mouse were randomly dividend into metastin group and control group.Both were injected with 2×106/ml.SUNE-1-5-8F cells under the right armpits.Mouse in metastin group were injected with 1.17 mg/kg metastin at day 2.The tumor inhibition rate,metastasis inhibition rate and life extension rate were detected by BALB/c-nu nude mouse model assay.The expression of kisspeptin protein was analyzed by immunohistochemistry. Results ①Metastin inhibited the SUNE-1-5-8F cells' capacity of metastasis and invasion in cell assay(P<0.05),whose capacity had a positive correlation with the concentration of metastin.②Metastin didn't inhibit the proliferation of tumor in nude mouse assay(P>0.05),but it reduced the pulmonary metastasis(P<0.05).And metastin influenced the life extension rate of nude mouse(P<0.05).The result of immunohistochemistry showed that the expression of kisspeptin protein of the metastin group was significantly different from the control group (P<0.05). Conclusions ①Metastin inhibits the SUNE-1-5-8F cells' capacity of metastasis and invasion in cell assay,whose capacity has a positive correlation with the concentration of metastin.②Metastin reduces the pulmonary metastasis and prolonged life,but it doesn't inhibit the proliferation of tumor in nude mouse assay.