小鼠胚胎脑源与脊髓源神经干细胞增殖能力的比较

目的 探讨小鼠前脑源和脊髓源神经干细胞 (NSCs) 的增殖能力差异.方法 选择孕14 d C57BL胎鼠, 机械分离法从前脑和脊髓分离培养神经干细胞, Nestin/Sox2免疫荧光染色鉴定NSCs.计算细胞倍增速率 (Multiplication rate) 和细胞倍增时间 (Cell doubling time) , Brd U/Nestin双标检测NSCs增殖.结果 由C57BL胎鼠前脑和脊髓分离得到的细胞群, 均可在体外分裂增殖形成神经球, Nestin&Sox2标记阳性.脑源NSCs增殖速度显著高于脊髓源 (P<0.05) , 而倍增时间则与增殖能力成反比;P5代时两种干细胞增殖最快;Nestin&Brd U双标的NSCs也证明了正在增殖的脑源NSCs (29.65±3.38) %显著多于脊髓源的NSCs (19.93±1.95) % (P<0.05) .结论 来源于前脑的NSCs增殖能力更强.

Comparison of Proliferation between Fetal Forebrain-and Spinal Cord-Derived Neural Stem Cells in Mice

Objective To discusses the differences of proliferation between fetal forebrain-and spinal cord-derived neural stem cells (NSCs) in mice.Methods The NSCs were separated by the mechanical method from the forebrain and spinal cord of 14 day C57 BL foetus, and the immunocyfluorescent staining of Nestin & Sox2 used to as the NSCs markers.Meanwhile, calculating multiplication rate and cell doubling time, with the addition of Brd U/Nestin double staining were used to test the proliferation.Results Firstly, multiplication rate of forebrain-derived NSCs significantly proliferated faster than spinal cord-derived (P<0.05) , and cell doubling time is inversely proportional to NSCs multiplication.Secondly, the 5 passages of these two sources of NSCs were the fastest.Lastly, the Brd U+ NSCs also suggested that the proliferating forebrain-derived NSCs (29.65%±3.38) were significantly more than spinal cord-derived (29.65%±3.38) (P<0.05) .Conclusion Our study suggests that the forebrain-derived NSCs have more capacity of proliferation.