顺铂耐药卵巢癌的差异表达基因和信号通路富集分析

目的 分析顺铂耐药卵巢癌的差异表达基因和信号通路.方法 用美国国立信息中心NCBI的GEO数据库获得顺铂耐药卵巢癌基因集GSE15372, 通过R与Bioconductor软件进行差异表达基因分析;再利用DAVID在线工具对顺铂耐药卵巢癌差异表达基因进行GO本体分析、KEGG通路分析.结果 差异倍数在2倍以上、FDR值小于0.05为标准, 筛选出上调差异表达基因和下调差异表达基因获得差异表达基因211个, 其中上调基因120个, 下调基因91个;基因富集分析发现差异表达基因集中在黏着斑、TGF-β信号通路等生物过程 (P<0.05) .结论 顺铂耐药卵巢癌存在一些特异的差异表达基因, 有部分信号通路在顺铂耐药卵巢癌中明显富集.

Analysis of Differential Expression Genes and Signal Pathways Associated with Cisplatin Resistant Ovarian Cancer

Objective To analyze the differentially expressed genes and signaling pathways in cisplatin resistant ovarian cancer. Me thods Cisplatin resistant ovarian cancer gene set GSE15372 was obtained from the GEO database of the national information center of the United States. The Differential expression gene analysis was performed by R and Bioconductor software, the GO and KEGG pathway of the differentially expressed genes were analyzed by DAVID online tool. Results 211 differentially expressed genes were obtained, including 120 up-and91 down-regulated genes as the standa rd was set that fold change was more than 2 times and FDR was less than0.05. Gene enrichment analysis of differentially expressed genes found in focal adhesion, TGF-beta signaling pathways and other biological processes (P<0.05) . Conclus ion There are some specific differentially expressed genes in the cisplatin resistant ovarian cancer, partial signal pathways were obvious enrichment in the cisplatin resistant ovarian cancer.