MPO和GSTM1、GSTT1基因多态性与儿童急性白血病易感性分析

目的 探讨髓过氧化物酶（myeloperoxidase，MPO）和谷胱甘肽S-转移酶（glutathione S-transferase，GST） M1、T1基因多态性及其交互作用与儿童急性白血病易感性的关系。方法 155名广东籍儿童急性白血病患者纳入病例组，155健康体检者为对照组。采用巢式聚合酶链式反应检测MPO （G-463A），GSTT1，GSTM1基因型。采用（口恶）2检验比较各基因型频率在病例组与对照组之间的差异，用OR及95%CI值表示各基因型发生急性白血病的危险度。结果 携带MPO-463位点A突变基因型（GA/AA）可能降低儿童急性白血病发病危险（OR=0.591，95%CI:0.356~0.981，P=0.041）；同时携带GSTT1 null基因和GSTM1 null基因的个体发生发生急性白血病的危险性是同时携带GSTT1 non-null基因和GSTM1non-null基因个体的2.991倍（95%CI:1.578~5.673）；同时携带MPO野生型（GG）基因及GSTT1 null基因和GSTM1 null基因进一步增加发病危险（OR=3.484，95%CI:1.626~7.466，P=0.041）。结论 同时携带MPO野生型（GG）及GSTT1 null基因和GSTM1 null基因的个体发生急性白血病的风险增大，可考虑作为儿童急性白血病易感性的重要生物标志物。

Analysis of genetic polymorphisms in MPO,glutathione S-transferase M1 and T1 and susceptibility to childhood acute leukemia

Objective To estimate the relationship between the genetic polymorphisms and interaction in MPO and glutathione S-transferase M1 and T1(GSTM1 and GSTT1)and the susceptibility to childhood acute leukemia. Methods 155 Guangdong children with acute leukemia and 155 healthy children were included in the case group and the control group,respectively. MPO, GSTM1 and GSTT1 genotypes were determined by multiplex polymerase chain reaction. A Chi-square test was used to compare the differences between the case group and the control group. Odds Ratio (OR) and 95% confidence interval (CI) were used to analyze the risk of acute leukemia in each genotype. Results MPO G-463A mutation genotype(GA and AA) was more likely to decrease risk of childhood acute leukemia(OR=0.591,95% CI:0.356-0.981, P=0.041); GSTT1 null and GSTM1 null genotype increased the risk of childhood acute leukemia 2.991-fold that of GSTT1 non-null and STM1 non-null genotype.The interaction of three genotypes showed further incident risk (OR=3.484, 95% CI:1.626-7.466,P=0.041). Conclusions The interaction of MPO G-463A GG genotype, GSTT1 null genotype and GSTM1 null genotype was related to an increased risk of childhood acute leukemia, which can be considered as an important biomarker to assess the susceptibility to childhood acute leukemia.