Roux-en-Y胃旁路术对大鼠肠道厚壁菌群及APPL1的影响

目的 探讨Roux-en-Y胃旁路术对肠道厚壁菌群及APPL1的影响.方法 使用Roux-en-Y胃旁路术作用于肥胖大鼠模型, 并观察术后以及术后粪菌移植对大鼠相关代谢指标的影响, 使用Western blot及q PCR检测不同分组的厚壁菌群及APPL1的表达.结果 手术后大鼠的体重较术前明显下降 (P<0.05) , 粪菌移植后体重有所回升, S+T组的体重较S组明显升高 (P<0.01) ;手术后大鼠的血浆中TG表达较术前明显下降 (P<0.01) , S+T组的血浆中TG表达较S组明显上升 (P<0.05) ;同时在骨骼肌中发现S组的TG表达明显低于C组, 在S+T组中的TG表达明显高于S组 (P<0.05) ;同时发现, 在S组中TCH的表达明显较C组低, 而S+T组的TCH表达较S组升高 (P<0.05) ;FINS在S组中的表达较C组明显下降, FINS在S+T组的表达较S组明显升高 (P<0.05) ;FGB在S组中的表达较C组明显下降, S+T组的FGB表达较S组明显上升 (P<0.05) ;ISI在S组的表达较C组明显上升 (P<0.05) , ISI在S+T组的表达组较S组明显下降 (P<0.01) .厚壁菌群在S的表达较C组明显降低 (P<0.05) .Western blot检测以及m RNA的检测发现APPL1在S组的表达较C组明显升高 (P<0.01) , S+T组中APPL1的表达较S组明显降低 (P<0.05) .结论 Roux-en-Y胃旁路术可能是通过影响厚壁菌群的表达量进而调节体重的.

Effects of Roux-en-Y Gastric Bypass on Intestinal Thickening Bacteria and APPL1 in Rats

Objective To investigate the effect of Roux-en-Y gastric bypass on intestinal thick-walled bacteria and APPL1.Methods The effect of postoperative and postoperative fecal transplantation on the metabolic index of rats was observed by Roux-en-Y gastric bypass.The thick-walled bacteria and APPL1 expression were detected by Western blot and qPCR.Results The body weight of the rats in the S+T group was significantly higher than that in the S group (P<0.05) .The body weight of the rats in the S+T group was significantly higher than that in the control group (P<0.05) The expression of TG in S+T group was significantly higher than that in S group (P<0.05) .The expression of TG in S group was significantly lower than that in group C (P<0.05) The expression of TCH in the T group was significantly higher than that in the S group (P<0.05) .The expression of TCH in the S group was significantly higher than that in the S group (P<0.05) The expression of FINS in S group was significantly higher than that in group C (P<0.05) .The expression of FINS in S group was significantly higher than that in group S (P<0.05) .The expression of ISI in S group was significantly higher than that in group S (P<0.05) .The expression of ISI in S group was significantly higher than that in group S (P<0.05) .The expression of S thick-walled bacteria in S was significantly lower than that in group C (P <0.05) .Western blot and mRNA detection showed that the expression of APPL1 in S group was significantly higher than that in group C (P<0.01) .The expression of APPL1 in S+T group was significantly lower than that in S group (P <0.05) .Conclus ion Roux-en-Y gastric bypass may be mediated by affecting the expression of thick-walled flora.