KL-6粘糖蛋白在百草枯中毒致肺纤维化大鼠中的表达及意义

目的 探讨KL-6(Krebs von den Lungen-6)粘糖蛋白在百草枯中毒致肺纤维化大鼠中的表达及意义。 方法 将雄性SD大鼠70只，采用随机平行对照法分成百草枯中毒1、4、7、14、21、28 d组和生理盐水对照组，每组10只，将1 ml浓度为20%的百草枯水溶液用无菌等渗盐水稀释成100 ml，中毒组按18 mg/kg剂量一次性腹腔注射给药，对照组予以等量生理盐水腹腔注射。排除因腹腔注射药物后不符合建模标准及死亡的大鼠，每组选取6只在染毒后1、4、7、14、21、28 d时间点进行处死，分别观察肺组织的病理变化、Masson染色观察肺组织纤维化变化、酶联免疫吸附法(Elisa)检测血清中KL-6水平、免疫组化检测肺组织中KL-6的表达变化。 结果 正常对照组SD大鼠血清中KL-6含量较低约为11.42 ng/ml，中毒4、7、14、21 d组大鼠血清中KL-6蛋白水平逐渐升高并均高于正常对照组(P<0.05)，28 d数值较21 d前下降，但较对照组均升高，差异有统计学意义。大鼠肺组织1、4、7、14、21 d组中KL-6表达较正常对照组显著升高(P<0.05)，28 d组数值较21 d组前下降，但较对照组均升高，差异有统计学意义(P<0.05)。 结论 KL-6随着百草枯中毒肺纤维化的程度变化而变化，其可能参与百草枯中毒大鼠肺纤维化过程。 

Expression and significance of KL-6 mucin in paraquat-induced murine pulmonary fibrosismodel

Objective To investigate the expression and significance of Krebs von den Lungen-6(KL-6) mucin in rats with pulmonary fibrosis induced by paraquat poisoning. Methods Seventy male SD rats were randomly divided into four groups:paraquat poisoning 1,4,7,14,21 and 28 d group and normal saline control group.1 ml paraquat aqueous solution with the concentration of 20% was diluted with sterile isotonic saline into 100 ml,and the poisoning group was administered intraperitoneally with 18 mg/kg,the control group was given the same amount of saline intraperitoneal injection.The rats with intraperitoneal injection of drugs that did not meet the modeling standards and died were excluded,after 1,4,7,14,21 and 28 d injection,6 rats were killed in the poisoned group respectively,and 6 rats were killed in the control group,respectively.The pathological changes of lung tissue were observed.The changes of lung tissue fibrosis were observed by Masson staining.The level of KL-6 in serum was detected by enzyme-linked immunosorbent assay(ELISA) in the expression of KL-6. Results The level of KL-6 in serum of normal control group was about 11.42 ng/ml,the level of KL-6 protein in the serum of 4,7,14 and 21 d group gradually increased and was higher than that in the normal control group(P<0.05),and decreased after 21 days while they were higher than the control group.The expression of KL-6 in the lung tissue of 1,4,7,14 and 21 was significantly higher than that in the normal control group(P<0.05),and decreased after 21 days,but they were always higher than the control group. Conclusion KL-6 changes with the degree of pulmonary fibrosis in paraquat poisoning,which may be involved in the process of pulmonary fibrosis in paraquat poisoning rats.