磁共振扩散加权成像与宫颈癌细胞增殖活性PCNA、Ki-67表达相关性的研究

目的 探讨子宫颈癌患者不同分化程度磁共振扩散加权成像(DWI)ADC值及rADC值的变化及其与宫颈癌细胞增殖活性PCNA、Ki-67的相关性。 方法 经病理确诊为宫颈癌的患者40例,所有患者术前均行磁共振检查并进行扩散加权成像;术中病理均进行PCNA以及Ki-67免疫组化染色。应用方差分析分析不同分化程度宫颈癌患者PCNA以及Ki-67表达情况,Pearson相关分析法分析PCNA以及Ki-67、ADC与rADC值的相关性。运用SPSS 20.0统计学软件对数据进行统计学分析,P<0.05为差异有统计学意义。 结果 低分化组ADC值以及rADC值低于高分化组,差异有统计学意义(t=2.118,P<0.05)。低分化组与中分化组之间差异无统计学意义(P>0.05)。随着分化程度降低,PCNA以及Ki-67表达程度逐渐升高。随着PCNA以及Ki-67表达程度的升高,ADC值与rADC值降低,差异均有统计学意义(P<0.05)。Pearson相关性检验后发现,ADC值与Ki-67和PCNA表达程度呈负相关(P<0.05)。 结论 宫颈癌DWI成像ADC、rADC值的测量有助于评价并鉴别宫颈癌不同病理级别并有潜力作为评价肿瘤增殖程度的无创性方法。 

The correlation between diffusion weighted imaging with cervical cancer cell proliferation activity of PCNA and Ki-67

Objective To evaluate the difference of diffusion weighted imaging (DWI) ADC value and rADC value of different degree of differentiation cervical cancer and correlation with cervical cancer cell proliferation activity (PCNA and Ki-67). Methods Forty patients with pathological diagnosis for cervical cancer were selected for this study.Preoperative magnetic resonance imaging (MRI) and DWI was performed before operation.Meanwhile,the PCNA and Ki-67 immunohistochemical staining were performed.The expression of PCNA and Ki-67 in different differentiation degree of cervical cancer were analysed by analysis of variance,and the correlation with ADC and the rADC value were also analysed by Pearson correlation methods.SPSS 20.0 was used to calculated the relevant data and significance was set at <0.05. Results The ADC values and rADC value in poorly differentiated group is less than high differentiation group,and the difference was statistically significant (t=2.118,P<0.05).There was no significant difference between the poorly differentiated group and middle differentiated group (P>0.05).With the degree of differentiation is reduced,the expression of PCNA and Ki-67 is increased.With the increase of the degree of expression of PCNA and Ki-67,ADC values and the rADC value decreased,the difference had statistical significance (P<0.05).Pearson correlation test showed that ADC values to be inversely associated with the degree of Ki-67 and PCNA expression. Conclusion DWI imaging and the ADC,rADC value measurement for cervical cancer can help to evaluate the cervical cancer with different pathological level and has the potential to be a non-invasive method for identifying tumor proliferation.