上转换发光技术侧流免疫夹心法检测59例麻风患者和87例家庭接触者PGL-I抗体结果分析

目的：评估上转换发光技术侧流免疫夹心法（UCP-LFA）检测PGL-I抗体对麻风接触者发病风险预测的价值。方法：采用UCP-LFA 方法检测贵州59例麻风患者、87例家庭接触者和55例健康对照进行PGL-I抗体水平。数据统计采用SPSS 20.0进行卡方分析。根据ROC曲线确定临界值。结果：麻风患者中PGL-I抗体阳性率为81.4%、麻风接触者为5.7%、健康对照人群为1.8%,三组人群PGL-I抗体阳性率差异有统计学意义（χ2 =126.47，P<0.005）。少菌型和多菌型PGL-I抗体阳性率分别为40% 和89%，差异有统计学意义（χ2=13.386，P<0.005）。BI+、BI-患者PGL-I抗体阳性率分别为83%和0%，差异有统计学意义（χ2 =17.560，P<0.005）。结论：麻风患者PGL-1抗体阳性率>麻风病接触者>正常对照，多菌型PGL-1抗体阳性率高于少菌型，提示PGL-I IgM抗体水平对麻风发病有一定的预测作用。

Detection of PGL-I antibody in 59 patients with leprosy and 87 household contacts by lateral flow immuno-sandwich assay using up-converting phosphor reporter particles

Objective: To evaluate the value of lateral flow immuno-sandwich assay using up-converting phosphor reporter particles (UCP-LFA) in predicting the risk of leprosy contacts by detecting PGL-I antibody. Methods: The PGL-I IgM level were measured by UCP-LFA in 59 patients with leprosy, 87 household contacts and 55 healthy controls in Guizhou. Data statistics were analyzed by using SPSS 20.0 for chi-square test. The?critical value was identified through receiver operating characteristic curve. Results: The positivity rate of anti-PGL-I IgM in patients, contacts and healthy controls were 81.4%, 5.7% and 1.8%, with significant differences between them (χ2 =126.47，P<0.005). The positivity rates of anti-PGL-I IgM in patients with paucibacillary leprosy (PB) and multibacillary (MB) were 40% and 89%, with a significant difference (χ2=13.386，P<0.005).The positivity rates of anti-PGL-I IgM in patients with BI+ and BI- were 83% and 0%, with a significant difference (χ2 =17.560，P<0.005). Conclusion: The PGL-1 IgM antibody can predict the risk of leprosy.