视网膜色素变性Rd1小鼠眼球中增殖细胞核抗原（PCNA）的表达

目的　探讨增殖细胞核抗原（proliferating cell nuclear antigen，PCNA）在Rd1小鼠视网膜上的表达情况。方法　取出生后14 d、21 d、28 d的Rd1小鼠和C57BL/6J小鼠各5只，摘出眼球进行HE染色，观察视网膜形态学结构变化；免疫组织化学染色与实时荧光定量PCR检测PCNA蛋白和基因在不同鼠龄小鼠中的表达。结果　HE染色结果显示:PCNA蛋白均在两种小鼠视网膜神经节细胞中表达；C57BL/6J小鼠视网膜结构完整；与C57BL/6J小鼠相比，Rd1小鼠视网膜发育随着鼠龄的增加，发生进行性退化。免疫组织化学染色结果显示:PCNA蛋白表达仅局限于视网膜神经节细胞，免疫阳性信号出现在第14天和第21天，而在第28天未检测到。PCR 实验结果显示:与C57BL/6J小鼠相比较，鼠龄21 d时Rd1小鼠PCNA基因表达水平增高，是C57BL/6J小鼠的2.23倍；鼠龄14 d和28 d时 Rd1小鼠PCNA基因表达均低于C57BL/6J小鼠（均为P<0.05）。结论　PCNA基因参与了Rd1小鼠视网膜发育退化过程。

The expression of proliferating cell nuclear antigen in Rd1 mouse with retinitis pigmentosa

Objective　To investigate the proliferating cell nuclear antigen（PCNA） expression in Rd1 mouse retina.Methods　The retina of 5 Rd1 mice （experimental group） and C57BL/6J mice （control group） from 14 d，21 d，28 d after birth were stained with HE.observed retinalmorphological structure changes.Immunohistochemical and real-time quantitative PCR （RT-QPCR） detection of PCNA protein and gene expression in the retina in different age mice.Results　The results of HE staining showed that PCNA protein was expressed in the retinal ganglion cells of both mice；the retinal structure of C57BL/6J mice was intact；and the retinal development of Rd1 mice degenerated progressively with the increase of age compared with C57BL/6J mice.Immunohistochemical staining showed that the expression of PCNA protein was confined to optic ganglion cells.Immunopositive signals appeared on the 14th and 21st days，It was not detected on the 28th day.Compared with C57BL/6J mice，the expression level of PCNA gene in Rd1 mice was 2.23 times higher than that in C57BL/6J mice at 21 days of age and lower than that of C57BL/6J mice at 14 and 28 days of age （all P<0.05）.Conclusion　PCNA gene is involved in the process of retinal degeneration inRd1 mice.