NLRP3炎性体在2型糖尿病合并糖尿病足患者中的作用

目的 探讨NLRP3炎性体在2型糖尿病合并糖尿病足患者中的作用。 方法 自2016年6月-2017年6月,前瞻性收集丽水市第二人民医院收治的2型糖尿病合并糖尿病足患者100例作为观察组,同期收集2型糖尿病患者50例(无糖尿病足)作为对照组。观察2组患者NLRP3炎性体表达情况。另外收集观察组患者主要临床特征,分析NLRP3炎性体与2型糖尿病足患者临床特征的相关性。 结果 与对照组比较,观察组患者NLRP3炎性小体mRNA相对表达量显著增高(4.12±0.88 vs. 2.73±0.92,P<0.001);NLRP3炎性小体蛋白质相对表达量显著增高(12.48±2.81 vs. 9.89±2.65,P<0.001)。糖尿病严重程度分级为0~1、2~3和4~5级的患者NLRP3炎性小体mRNA相对表达量分别为3.27±0.78、3.87±0.83、4.83±0.92,差异有统计意义(P=0.002);NLRP3炎性小体蛋白质相对表达量分别为9.65±2.12、11.32±2.54、15.49±2.98,差异有统计学意义(P<0.001)。Pearson线性相关性分析显示NLRP3炎性小体mRNA相对表达量、NLRP3炎性小体蛋白质相对表达量均与糖尿病足严重程度评分、糖化血红蛋白浓度成正相关(P<0.05)。 结论 2型糖尿病合并糖尿病足患者NLRP3炎性体激活,与糖尿病足的发生发展过程有关。 

The role of NLRP3 inflammasome in patients with type 2 diabetes with diabetic foot

Objective To investigate the role of NLRP3 inflammasome in type 2 diabetes mellitus with diabetic foot. Methods From June, 2016 to June, 2017, 100 patients with type 2 diabetes mellitus and diabetic foot treated in our hospital were selected as an observation group, and 50 patients with type 2 diabetes mellitus (without diabetic foot) were selected as a control group. The expression of NLRP3 between the two groups were observed. In addition, the main clinical features of the patients in the observation group were collected, and the correlation between the NLRP3 inflammasome and the clinical features of diabetic foot were analyzed. Results When compared with the control group, the relative expression of mRNA of the NLRP3 inflammasome increased significantly in the observation group(4.12±0.88 vs. 2.73±0.92, P<0.001), and the relative expression level of NLRP3 inflammasome protein increased significantly(12.48±2.81 vs. 9.89±2.65, P<0.001). The NLRP3 inflammasome mRNA expression in patients of 0-1, 2-3 and 4-5 Diabetes severity grading were 3.27±0.78, 3.87±0.83 and 4.83±0.92, the difference was statistically significant (P=0.002). NLRP3 inflammasome protein expression levels were 9.65±2.12, 11.32±2.54 and 15.49±2.98, the difference was statistically significant (P<0.001). Pearson linear correlation analysis showed that the relative expression level of NLRP3 mRNA and the relative expression of NLRP3 inflammasome protein were all directly proportional to the diabetic foot severity score and glycosylated hemoglobin (P<0.05). Conclusion The activation of NLRP3 inflammasome in patients with type 2 diabetes mellitus combined with diabetic foot is related to the development of diabetic foot.