头部X线照射后大鼠血清中糖皮质激素含量及脾细胞中糖皮质激素受体变化机制的研究

观察头部Ｘ线照射后血清皮质酮含量和脾细胞中糖皮质激素受体的变化及其机制。给Ｗｉｓ－ｔａｒ大鼠以１０ＧｙＸ线头部照射后不同时间测定其血清皮质酮含量和脾细胞中糖皮质激素受体。结果表明：头部Ｘ线照射后皮质酮含量的变化呈双峰曲线，第１天即显著增高，至第９天仍显著增高；头部Ｘ线照射后２４小时受体数已显著降低，至第９天恢复近正常，受体和皮质酮含量的变化规律无相关性；体外实验也证实内源性糖皮质激素含量的增高不是引起受体变化的主要原因；头部Ｘ线照射后脾脏中Ｂ淋巴细胞数的减少与糖皮质激素受体的变化规律相同，而Ｔ淋巴细胞增多，说明脾细胞中糖皮质激素受体的变化可能和Ｔ、Ｂ淋巴细胞比例的变化有关；头部Ｘ线照射后受体的最大结合容量有降低趋势，但差异不显著，而平衡解离常数（Ｋｄ）值显著增大，亲和力降低，说明受体数的减少是亲和力降低的结果。作者提出，在应激反应中存在复杂的神经内分泌免疫调节系统，只有综合考虑激素和受体等多方面的改变，才能对应激反应作出正确的分析。

Changes of serum corticosterone and glucocorticoid receptors in rat splenocytes after Xray irradiation on the head

e measured the serum corticosterone (CS) content and glucocorticoid receptors (GCR) in splenocytes after lo Gy Xray irradiation on the heads of Wistar rats.The timecourse curve of serum CS showed a double peak curve.It increased significantly one day after irradiation and still remained high on the 9th day.The GCR decreased significantly 24 hr after irradition,and then increased slowly until the 9th day when it approached to the control level.The pattern of GCR change was different from that of serum CS content.The time course of GCR chang was similar to that of B lymphocytes.The change of T lymphocytes in the spleen was in the opposite direction.The data suggest that the GCR change is more likely related to the change of lymphocyte subpopulation rather than interference by endogenous CS.Bmax tended to decrease after head irradiation,but the difference was not significant statistically,while the Kd value increased significantly and the affinity decreased.This demonstrated that GCR reduction was due to decreased affinity.It is suggested that a complicated neuroendocrinoimmunoregulatory system is implicated in stress.