实验性重症急性胰腺炎肺内IL—1β及IL—18mRNA的表达

目的：观察实验性重症急性胰腺炎（severe acute pancreatitis,SAP）肺内IL－1β及IL－18mRNA的表达，并探讨其与肺损伤的关系，方法：SD大鼠32只，随机分4组：正常对照组、SAP6h组、SAP12h组、SAP18h组。采用5％牛磺胆酸钠（0.1ml/100g）胆胰管内逆行注射诱导大鼠SAP模型。血清淀粉酶采用HITACHI－7150型自动生化分析仪测定；半定量RT－PCR检测肺组织内IL－1β及IL18mRNA的表达，结果：造模后各时间点血清淀粉酶水平显升高，12h达到高峰，与正常对照组相比，均具有显性差异（P＜0．01）。正常肺组织内可见IL－1β及IL－18mRNA表达；SAP各组肺组织内IL－1β及IL－18mRNA的表达明显增强，与正常对照组比较均有显性差异（P＜0．01），结论：除TNF-α外，肺内生成过多的IL－1β及IL－8在SAP并发急性肺损伤（acute lung injury,ALI）及急性呼吸窘迫综合征（acute respiratory distress sysdrome,ARDS)进程中可能发挥重要的作用。

Intrapulmonary expression of IL-1B and IL-18 mRNA in experimental severe acute pancreatitis.

Objective To investigate the intrapulmonary expressions of IL-1B and IL-18 mRNA, and to detect the correlation of such expressions with lung injury in experimental severe acute pancreatitis (SAP). Methods Thirty two SD rats were randomized into four groups: normal controls (NC), SAP 6 h, SAP 12 h and SAP 18 h. SAP was induced by retrograde infusion of 5% sodium taurocholate (0. 1 ml/100 g)into the bili-pancreatic duct. Serum amylase was measured by using an automatic HITACHI-7150 analyzer. Intrapulmonary expressions of IL-1B and IL-18 mRNA were detected by semi-quantitative RT-PCR. Results Serum amylase levels were significantly increased after the induction of pancreatitis, reaching the peak at 12 h, with statistical significance compared with the NC group (P<0. 01). Expressions of IL-1J3 and IL-18 mRNA were observed in the lungs of normal rats. Intrapulmonary expressions of IL-1B and IL-18 mRNA were increased greatly during taurocholate-induced SAP, with statistical significance compared with the NC group(P<0. 01). Conclusions In addition to TNFa, overproduction of IL-1B and IL-18 in the lungs might play an important role in the course of ALI and ARDS during the experimental SAP.