应用末端脱氧核苷酰转移酶法研究化疗药诱导人肿瘤细胞的凋亡

应用末端脱氧核苷酰转移酶法（ＴｄＴ法）研究抗肿瘤药物诱导人肿瘤细胞的凋亡。方法应用ＴｄＴ法、ＤＮＡ凝胶电泳以及电镜、光镜等技术研究有关细胞的凋亡。结果本研究表明，顺铂可以诱导人白血病ＨＬ－６０细胞和经ＳＶ４０－Ｔ转染的人支气管上皮细胞的凋亡，并有很好的剂量和时间反应，同时还观察到，这些细胞的ｂｃｌ－２ｍＲＮＡ表达降低先于细胞凋亡的发生。应用ＴｄＴ法检测，在卡铂治疗的卵巢癌患者的肿瘤组织中观察到了凋亡细胞。结论ＴｄＴ法有可能用于检测经化疗诱导的人肿瘤细胞的凋亡，以及评价治疗过程中肿瘤细胞对治疗的反应。

Study on antitumor drug-inducedapoptosis in human cancer cells by terminaldeoxynucleotidyl trans-ferase assay

Objective Considerable evidence has showed that apoptosis is involved in both can-cer development and inhibition.A new assay (terminaldeoxynucleotidyl transferase assay,TdT as-say)ws recently reported to have advantages in the detection of apoptosis,In this study,this assay wasused to investigate antitumor drug-induced apoptosis in human cancer cells.Methods TdT as-say,DNA gel electrophoresis,electron and light microscopy were used to observe apoptosis. Results Our results showedthatcisplatin-induced apoposis in both HL-60 and SV40T-transformed human bronchial epithelial cells was detected with a good dose and time response.The occurrence of the apoptosis was priceded by the decrease of bcl-2 mRNA exression.With TdT assay,apoptotic cells were obsrerved in the ovarian tumor of patients treated with carboplatin.Conclusion TdT assay may be applicabe to moniter apoptosis in human cancers induced by chemotherapy,and to evaluate tumor cell uesponse during treatment.