Interleukin-12 gene modification exerts anti-tumor effects on murine mammary sarcoma cell line in vivo |
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Authors: | Li Dan Yu Hong Xu Teng Fei Li Jing Hua Sun Yun Fang Zhang Wen Qing |
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Institution: | [1]Department of Microbiology, Medical College of Qingdao University, 308 Ningxia Road, Qingdao 266071, China [2]Department of Central Laboratory, Qingdao Municipal Hospital, 1 JiaozhouRoad, Qingdao 266011, China [3]Department of Clinical Laboratory Medicine, Shandong Medical College,39 Qingnian Road, Linyi 276002, China [4]Department of Microbiology, Medical College of Qingdao University, 308 Ningxia Road, Qingdao 266071, China. |
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Abstract: | The aim of this project was to investigate the anti-tumor effect of an IL-12 gene modified mammary sarcoma murine cell line, EMT6/IL-12, in mouse model. In this study, we transfected the recombinant eukaryotic plasmid encoding IL-12 gene (pcDNA6-p70) into EMT6 and obtained the IL-12 expressing EMT6/IL-12 cell line. Then EMT6/IL-12 cells were s.c. inoculated into mice. The recombinant vector treatment group was set as control. We then evaluated the inhibition of tumor growth and the anti-tumor immunity function in vivo such as cytotoxicity, proliferation of splenocytes and serial IFN-gamma level. And the percentage of IFN-gamma producing CD4 or CD8 T cells among splenocytes was also analyzed in tumor bearing mice. Our results showed that the growth of tumors was obviously inhibited in EMT6/IL-12 group. Moreover, the capacities of anti-tumor immunity were all significantly higher in EMT6/IL-12 group compared to the controls. The results of the present investigation support the notion that EMT6/IL-12 could exert gene therapy in tumor model by improving the anti-tumor cellular immunity. |
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Keywords: | interleukin-12 transformation genetic gene therapy mammary neoplasm |
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