针刺对小鼠腹腔巨噬细胞的热休克蛋白、诱导性一氧化氮合酶及其基因表达的效应

目的研究针刺对小鼠腹腔巨噬细胞的热休克蛋白（ＨＳＰ）、诱导性一氧化氮合酶（ｉＮＯＳ）及其ｍＲ－ＮＡ表达的效应。方法将２４只昆明小鼠腹腔注射无菌石蜡油后，随机分为３组：电针（ＥＡ）组、对照１（Ｃ１）组和对照２（Ｃ２）组。将３组收集的腹腔巨噬细胞制备成玻片和硝酸纤维素膜（ＮＣＭ）两种标本，应用原位杂交、免疫细胞化学、细胞化学及斑点印迹技术检测ＨＳＰ７０、ｉＮＯＳ及ｉＮＯＳｍＲＮＡ。结果ＨＳＰ７０定位于巨噬细胞的胞质和胞核；ｉＮＯＳｍＲＮＡ及ｉＮＯＳ均定位于巨噬细胞的胞质；３组的ｉＮＯＳｍＲＮＡ、ｉＮＯＳ、ＨＳＰ７０的斑点印迹的扫描数值均为ＥＡ组＞Ｃ２组＞Ｃ１组（Ｐ＜０．０１）。结论电针可显著提高小鼠腹腔巨噬细胞的ＨＳＰ７０、ｉＮＯＳ及ｉＮＯＳｍＲ－ＮＡ表达。

ACUPUNCTURE EFFECTS ON INDUCIBLE NITRIC OXIDE SYNTHASE mRNA, iNOS PRODUCT AND HEAT SHOCK PROTEIN IN PERITONEAL MACROPHAGES OF THE MOUSE

Objective\ To study the acupuncture effects on the inducible nitric oxide synthase (iNOS) mRNA, iNOS product and heat shock protein(HSP) 70 in the mouse macrophages. Methods\ After peritoneal stimulation with steriled paraffin oil for 48h, 24 Kunming mice were randomly divided into 3 groups. They included:electroacupuncture(EA) group, treated with EA; Control 1(C1) group, peritoneal macrophages treated with culture medium for 18h; and control 2(C2) group, treated with neither EA nor culture The macrophages in 3 groups collected from respective peritoneal cavity were prepared into two kinds of specimens including slide and nitrocellulose membrane (NCM) specimens. The iNOS mRNA, iNOS and HSP70 were detected respectively with in situ hybridization, cytochemistry, immunohistochemistry and RNA and protein dot blots. Results\ The results showed that the signals of iNOS mRNA and iNOS product were localized in the macrophage cytoplasm; the immunoreactivity (IR) of HSP70 localized in both cytoplasm and nucleus. The dot blot signal scanning value of those in 3 groups in comparison all showed as follows: EA group>C2 group >C1 group, P<0 01 .Conclusion\ Acupuncture effect could up regulate expression of iNOS mRNA and promote iNOS activity and immunoreactivity of HSP70.