姜黄素对脂多糖诱导腹腔巨噬细胞释放炎症因子的影响

目的探讨姜黄素对脂多糖(lipopolysaccharide,LPS)刺激腹腔巨噬细胞(peritoneal macrophages,PMs)分泌炎症因子的影响。方法分离、培养腹腔巨噬细胞,用不同浓度姜黄素(12.5、25、50 mg/L)预处理细胞12 h,进而使用100ng/ml LPS刺激细胞,12 h后收集培养基上清液,ELISA检测促炎介质肿瘤坏死因子(tumor necrosis factor-α,TNF-α)、白介素6(interleukin-6,IL-6)的含量;30 min后收集细胞裂解物,Western blot检测p38 MAPK激酶活性。结果未经处理的腹腔巨噬细胞经LPS刺激12 h后,上清中TNF-α、IL-6的表达水平显著上升(p<0.05);12.5 mg/L姜黄素对LPS诱导的TNF-α、IL-6表达无显著影响(p>0.05);而50、25 mg/L姜黄素可以明显抑制LPS诱导的TNF-α、IL-6的表达(p<0.05);且25 mg/L姜黄素能够明显抑制LPS激活的p38 MAPK激酶的磷酸化水平。结论一定浓度的姜黄素可以抑制LPS刺激腹腔巨噬细胞炎症因子TNF-α、IL-6的分泌,进而减轻炎症反应的程度。

EFFECT OF CURCUMIN ON LPS-INDUCED PERITONEAL MACROPHAGES RELEASE OF INFLAMMATORY FACTORS

Objective To investigate the effect of curcumin on inflammatory cytokine release by peritoneal macrophages （PMs） stimulated by lipopolysaccharide （LPS）. Method PMs were isolated and cultured, then pretreated with different concentra- tions of curcumin （ 12. 5,25,50 mg/L） for 12 h followed by 100 ng/ml LPS stimulation. TNF - ct and IL -6 in the cell supernatants were detected by ELISA after 12 h LPS stimulation; the activity of p38 MAPK in the cell lysates was investigated by Western blot after 30 min LPS stimulation. Result TNF- ct and IL- 6 levels increased dramatically after 12 h stimulation in the control group （p 〈 0. 05） ; and 12. 5 mg/L curcumin had no effect on TNF -α and IL -6 expression （p 〉0. 05）. However, both expression can be in- hibited by 50 and 25 mg/L curcumin （p 〈0. 05 ）. Furthermore, the phosphorylation of p38 MAPK can be inhibited by 25 mg/L cur- cumin. Conclusion Certain concentrations of curcumin can inhibitor the release of TNF -α and IL - 6 by PMs stimulated by LPS, which can further alleviate inflammation.