周围型肺癌HRCT征象与骨桥蛋白水平的相关性研究

目的:确定不同类型周围型肺癌的肺癌骨桥蛋白(osteopontin,OPN)值的表达是否有差异及肺癌患者癌组织内OPN与肿瘤的影像分期的相关性。方法:经皮CT穿刺活检并经病理确诊的肺癌组织标本143例。石蜡切片脱蜡、水化后采用柠檬酸盐缓冲液高温高压抗原修复法。采用SP免疫组织化学检测OPN的表达。高倍视野下,观察阳性细胞染色强度,计数阳性细胞百分数,按着色细胞百分数计分,确定OPN阳性或阴性。结果:OPN在肺癌组织中有较强的阳性表达率。阳性染色主要位于细胞膜上,胞浆中亦有少量表达。肺癌组织中OPN的表达与肿瘤的分化程度和临床分期相关(P<0.05),而与病理组织类型无关(P>0.05)。结论:OPN可作为临床TNM分期的主要生化指标,可作为判断肺癌患者的预后及术后复发的参考指标。

Study on HRCT signs of peripheral lung cancer and the level of osteopontin protein correlation

Objective:To determine the different types of peripheral lung cancer if expression of OPN values differ,among patients with lung cancer within the OPN and tumor stage image correlation.Methods: Percutaneous CT biopsy and pathological specimens were diagnosed in 143 cases.Paraffin dewaxing,Hydration,the use of high pressure and temperature citrate buffer antigen retrieval method was used.By SP immuno-histochemistry expression of OPN.and high power field microsccopy,the tissues were observed for staining intensity of positive cells,counting the percentage of positive cells by scoring the percentage of colored cells to determine the OPN positive or negative.Results: OPN have strong positive expression in lung cancer.Positive rate located mainly in the cell membrane,cytoplasm,and a few expressions.Imaging features of lung cancer and the expression of OPN in the share differences.Conclusion: OPN can be used as the main clinical TNM staging,and biochemical indicators may serve as the prognosis of patients with lung cancer and recurrence of reference.