| 鳗鲡迟缓爱德华氏菌偶联疫苗免疫效果评价 |
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| 引用本文： | 潘鸿洧,石存斌,赵毅,刘坡,吴淑勤. 鳗鲡迟缓爱德华氏菌偶联疫苗免疫效果评价[J]. 免疫学杂志, 2010, 0(2) |
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| 作者姓名： | 潘鸿洧 石存斌 赵毅 刘坡 吴淑勤 |
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| 作者单位： | 中国水产科学研究院珠江水产研究所;石河子大学动物科技学院;陕西省动物疾病控制中心; |
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| 基金项目： | “十一五”国家科技支撑计划课题“商品化渔用疫苗研制和规模化应用技术”项目(2006BAD03B05) |
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| 摘 要： | 目的迟缓爱德华氏菌是目前水产养殖中主要的病原菌,其感染谱甚广,危害巨大。因此,研制能有效预防这种致病菌的疫苗,将会极大的促进水产养殖业的发展。方法用OMP-LPS偶联物、OMP、LPS、FKC(福尔马林灭活全菌)以及生理盐水对照组按相同程序分别免疫鳗鲡。通过溶菌酶活性检测、微量凝集反应试验,结果鳗鲡经OMP-LPS偶联物、OMP、LPS以及FKC疫苗免疫后,各免疫组间血清中溶菌酶活性没有显著差异(P0.05),但均远高于生理盐水对照组(P0.05);OMP-LPS偶联组抗迟缓爱德华氏菌(E.tarda)E29L株的抗体效价较单纯的LPS组、OMP组,FKC组抗体滴度高,且持续时间长;对迟缓爱德华氏菌(E.tarda)E29L株的攻击,OMP-LPS偶联组保护率为84%,高于单纯OMP组的80%,LPS组的44%和FKC组60%。结论 OMP-LPS偶联物、OMP、LPS、FKC添加佐剂ISA763,各组的溶菌酶活性均显著高于不添加佐剂各组(P0.05),除了FKC+ISA763佐剂组外,其余各组均与不添加佐剂各组差异极显著(P0.01),且添加佐剂各组保护率都明显高于不添加佐剂各组,说明添加ISA763佐剂能够更好的启动鱼类的特异性和非特异性免疫。
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| 关 键 词： | 迟缓爱德华氏菌 鳗鲡 外膜蛋白 脂多糖 偶联疫苗 |
Immunogenic evaluation of the conjugated subunit vaccine against Edwardsiella tarda |
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| PAN Hongwei,SHI Cunbin,ZHAO Yi,LIU Po,WU Shuqin Pearl River Fisheries Research Institute,Chinese Academy of Fishery Science,Guangzhou ,China. Immunogenic evaluation of the conjugated subunit vaccine against Edwardsiella tarda[J]. Immunological Journal, 2010, 0(2) |
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| Authors: | PAN Hongwei SHI Cunbin ZHAO Yi LIU Po WU Shuqin Pearl River Fisheries Research Institute Chinese Academy of Fishery Science Guangzhou China |
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| Affiliation: | PAN Hongwei,SHI Cunbin,ZHAO Yi,LIU Po,WU Shuqin Pearl River Fisheries Research Institute,Chinese Academy of Fishery Science,Guangzhou 510380,China |
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| Abstract: | Edwardsiella tarda is an important pathogen in the aquiculture now,and developing an effective vaccine to prevent this pathogen become necessary.In this study,eels were respectively immunized with the OMP-LPS conjugates,OMP,LPS,and FKC in the experimental group,and immunized with physiological salt solution in the control group in the same procedure.Then lysozyme activity test and micro-agglutination test were performed.There was no significant difference of lysozyme activity(P>0.05) among experimental grou... |
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| Keywords: | Edwardsiella tarda Eel Outer membrane protein Lipopolysaccharide Conjugated vaccine |
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