脑缺血早期大脑皮质和尾壳核一氧化氮合酶神经元的进程变化

目的：探讨一氧化氮合酶神经元在脑缺血早期（２４ｈ内）的变化规律。方法：ＮＡＤＰＨ－ｄ组织化学方法。结果：各组间正常侧ＮＯＳ神经元无明显变化，散在分布于皮质的Ⅱ～Ⅵ层，以Ⅳ～Ⅵ为主，尾壳核内ＮＯＳ神经元数量较多，密集分布于外侧区。缺血１５ｍｉｎ和３０ｍｉｎ组，缺血中心区皮质和尾壳核ＮＯＳ神经元数量减少，形态无明显变化；缺血１ｈ和２ｈ组，皮质和尾壳核内ＮＯＳ神经元数量进一步减少，突起变短；缺血３ｈ和６

TEMPORAL CHANGES OF NITRIC OXIDE SYNTHASE NEURONS IN CEREBRAL CORTEX AND CAUDATE-PUTAMEN NUCLEI DURING EARLY STAGE OF CEREBRAL ISCHEMIA IN RATS

To study the rule of changes of NOS neurons during early stage (less than 24 h) of cerebral ischemia. Method: NADPH-d histochemical technique was used. Results: There were no noticeable changes of NOS neurons on the normal sides of the cerebral cortex in different animal groups, distributing in II - VI layers of the cortex, particularly in IV ~ VI layers, the NOS neurons in the caudate-putamen nuclei (CPu) were numerous in number, concentrating in their lateral part. In 15 min and 30 min ischemic groups, the number of NOS neurons at the ischemic core regions of the cortex and CPu were reduced. In 1 h and 2 h ischemic graups, the NOS neurons were further reduced, their processes became shorter in lenght. In 3h and 6 h ischemic groups, the NOS neurons were rare and their contours became irregular in shape, their processes were difficult to be found. In 12 h and 24 h ischemic groups, NOS neurons disappeared. Conclusion: NOS neurons in the cortex and CPu are more vulnerable to insults of cerebral ischemic.