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| 环孢霉素A合成抗原的单抗制备技术探讨 | |
| 引用本文： | Chen M,Fan X,Wei D,Wu Q,Feng P,Lü X.环孢霉素A合成抗原的单抗制备技术探讨[J].四川大学学报(医学版),2003,34(3):571-573. |
| 作者姓名： | Chen M Fan X Wei D Wu Q Feng P Lü X |
| 作者单位： | 1. 四川大学华西医院,传染科,成都,610041 2. 四川大学华西基础医学与法医学院,免疫学教研室 3. 四川大学化工学院 |
| 基金项目： | 四川省重点科学技术研究基金 ( 970 12 15 2 5 0 1),四川省学术和技术带头人培养基金,四川省卫生厅基金资助 |
| 摘 要： | 目的：研究利用自制的合成抗原制备环孢霉素A单克隆抗体的方法。方法：利用光化学反应将环孢霉素A与聚-L-赖氨酸偶联为合成抗原,免疫BALB／e小鼠,将免疫后小鼠脾细胞与小鼠骨髓瘤细胞SP2／0进行细胞融合,经反复筛选亚克隆获得抗CsA抗体的杂交瘤细胞株,以间接ELISA法进行抗体鉴定。结果：获得4株分泌抗CsA抗体的杂交瘤细胞株,其中2株分泌的抗体效价高,有一定特异性。结论：该抗原合成和免疫的方法切实可行,但单克隆抗体制备及保存方法有待进一步改进。 |
| 关 键 词： | 环孢霉素A 合成抗原 制备技术 单克隆抗体 |
| 修稿时间： | 2002年4月19日 |
On preparation the monoclonal antibody against synthetic antigen of cyclosporine A |
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| Chen Mina,Fan Xinjian,Wei Dapeng,Wu Qiongying,Feng Ping,Lü Xiaoju.On preparation the monoclonal antibody against synthetic antigen of cyclosporine A[J].Journal of West China University of Medical Sciences,2003,34(3):571-573. | |
| Authors: | Chen Mina Fan Xinjian Wei Dapeng Wu Qiongying Feng Ping Lü Xiaoju |
| Institution: | Department of Infectious Disease, West China Hospital, Sichuan University, Chengdu 610041, China. |
| Abstract: | Objective To study the methods for preparing the monoclonal antibody against synthetic antigen of cyclosporine A. Methods These included using photochemical reaction to couple cyclosporine A with poly-L-lysine as synthetic antigen, immunizing BALB/c mice, fusing spleen cells and SP2/0 cells. Then hybridoma cells were repeatedly screened and subcloned. The monoclonal antibodies were identified by indirect ELISA. Results Four hybridoma cells in secreting anti-cyclosporine A monoclonal antibodies were obtained. Only two showed high titer and some specificity. Conclusions The methods of antigen synthesis and immunization were practicable. But the methods of antibody preparation and preservation need to be improved. |
| Keywords: | Cyclosporine A Poly-L-lysine Synthetic antigen Monoclonal antibody |
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