四种消毒剂应用于DUWLs微生物污染控制的生物安全性评价

目的　评价四种不同消毒剂应用于牙科治疗椅水路系统(Dental Unit Waterlines, DUWLs)微生物污染控制的生物安全性。方法 通过CCK-8法研究四种不同的消毒剂：过氧化氢纳米银离子消毒剂（Sanosil）、次氯酸钠消毒剂（NaClO）、过氧化氢消毒剂(H2O2)和洗必泰（Chlorhexidine，CHX）在不同浓度时在体外对人口腔黏膜上皮细胞细胞毒性，比较不同消毒剂使用的生物安全性。结果 四种消毒剂在常用的消毒浓度下与人口腔上皮黏膜细胞作用一定时间下均可产生细胞毒性，但0.2%过氧化氢纳米银离子消毒剂和0.2%的洗必泰毒性等级为中度毒性（2级），5.25%次氯酸钠和3%过氧化氢毒性等级为重度毒性（4级）。结论 不同消毒剂在体外的细胞毒性不同，过氧化氢纳米银离子和洗必泰在牙科综合治疗台水路污染控制的常用浓度上对细胞的毒性低于次氯酸钠和过氧化氢，相对安全。

Biosafety evaluation of four disinfectants applied to microbial contamination control in dental unit waterlines

Objective　To evaluate the biosafety of four different disinfectants applied to microbial contamination control in DUWLs. Methods The cytotoxicity of four different disinfectants (hydrogen peroxide nano silver ion, sodium hypochlorite, hydrogen peroxide, chlorhexidine) on human oral mucosal epithelial cells under different concentrations in vitro and the biosafety of different disinfectants were studied and compared by CCK-8. Results The four disinfectants all produced cytotoxicity after being treated with human oral epithelial mucosal cells for a certain period of time under the common disinfection concentration. However, the toxicity grades of 0.2% hydrogen peroxide nano silver ion and 0.2% chlorhexidine were medium toxicity (Grade 2), the toxicity grades of 5.25% sodium hypochlorite and 3% hydrogen peroxide were severe toxicity (Grade 4). Conclusion The cytotoxicity of different disinfectants in vitro is different. Hydrogen peroxide nano silver ion and chlorhexidine are less toxic to cells than the sodium hypochlorite and hydrogen peroxide under the common concentration of water pollution control in dental unit waterlines and are relatively safe.