| Chaga mushroom (Inonotus obliquus) induces G0/G1 arrest and apoptosis in human hepatoma HepG2 cells |
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| 引用本文： | Myung-Ja Youn,Jin-Kyung Kim,Seong-Yeol Park,Yunha Kim,Se-Jin Kim,Jin Seok Lee,Kyu Yun Chai,Hye-Jung Kim,Hong Seob So,Ki-Young Kim,Raekil Park. Chaga mushroom (Inonotus obliquus) induces G0/G1 arrest and apoptosis in human hepatoma HepG2 cells[J]. World journal of gastroenterology : WJG, 2008, 0(4) |
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| 作者姓名： | Myung-Ja Youn Jin-Kyung Kim Seong-Yeol Park Yunha Kim Se-Jin Kim Jin Seok Lee Kyu Yun Chai Hye-Jung Kim Hong Seob So Ki-Young Kim Raekil Park |
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| 作者单位： | Vestibulocochlear Research Center Wonkwang University School of Medicine，Jeonbuk，Korea，Vestibulocochlear Research Center，Wonkwang University School of Medicine，Jeonbuk，Korea，Vestibulocochlear Research Center，Wonkwang University School of Medicine，Jeonbuk，Korea，Vestibulocochlear Research Center，Wonkwang University School of Medicine，Jeonbuk，Korea，Vestibulocochlear Research Center，Wonkwang University School of Medicine，Jeonbuk，Korea，Department of Bionanochemistry，College of Natural Science，Wonkwang University，Jeonbuk，Korea，Department of Bionanochemistry，College of Natural Science，Wonkwang University，Jeonbuk，Korea，Department of Family Medicine，Wonkwang University Hospital，Wonkwang University，Jeonbuk，Korea，Vestibulocochlear Research Center，Wonkwang University School of Medicine，Jeonbuk，Korea，College of Human Environmental Science，Wonkwang University，Jeonbuk，Korea，Vestibulocochlear Research Center，Wonkwang University School of Medicine，Jeonbuk，Korea |
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| 基金项目： | the Program for the Training of Graduate Students in Regional Innovation which was conducted by the Ministry of Commerce Industry and Energy of the Korean Government |
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| 摘 要： | AIM: To investigate the anti-proliferative and apoptotic effects of Chaga mushroom (Inonotus obliquus) water extract on human hepatoma cell lines,HepG2 and Hep3B cells. METHODS: The cytotoxicity of Chaga extract was screened by 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay. Morphological observation,flow cytometry analysis,Western blot were employed to elucidate the cytotoxic mechanism of Chaga extract. RESULTS: HepG2 cells were more sensitive to Chaga extract than Hep3B cells,as demonstrated by markedly reduced cell viability. Chaga extract inhibited the cell growth in a dose-dependent manner,which was accompanied with G0/G1-phase arrest and apoptotic cell death. In addition,G0/G1 arrest in the cell cycle was closely associated with down-regulation of p53,pRb,p27,cyclins D1,D2,E,cyclin-dependent kinase (Cdk) 2,Cdk4,and Cdk6 expression. CONCLUSION: Chaga mushroom may provide a new therapeutic option,as a potential anticancer agent,in the treatment of hepatoma.
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Chaga mushroom (Inonotus obliquus) induces G0/G1 arrest and apoptosis in human hepatoma HepG2 cells |
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| Myung-Ja Youn,Jin-Kyung Kim,Seong-Yeol Park,Yunha Kim,Se-Jin Kim,Hong Seob So,Raekil Park,Vestibulocochlear Research Center,Wonkwang University School of Medicine,Jeonbuk,Korea Ki-Young Kim,Ming-Xun Cui,College of Human Environmental Science,Wonkwang University,Jeonbuk,Korea Jin Seok Lee,Kyu Yun Chai. Chaga mushroom (Inonotus obliquus) induces G0/G1 arrest and apoptosis in human hepatoma HepG2 cells[J]. World journal of gastroenterology : WJG, 2008, 0(4) |
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| Authors: | Myung-Ja Youn Jin-Kyung Kim Seong-Yeol Park Yunha Kim Se-Jin Kim Hong Seob So Raekil Park Vestibulocochlear Research Center Wonkwang University School of Medicine Jeonbuk Korea Ki-Young Kim Ming-Xun Cui College of Human Environmental Science Wonkwang University Jeonbuk Korea Jin Seok Lee Kyu Yun Chai |
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| Affiliation: | Myung-Ja Youn,Jin-Kyung Kim,Seong-Yeol Park,Yunha Kim,Se-Jin Kim,Hong Seob So,Raekil Park,Vestibulocochlear Research Center,Wonkwang University School of Medicine,Jeonbuk,Korea Ki-Young Kim,Ming-Xun Cui,College of Human Environmental Science,Wonkwang University,Jeonbuk,Korea Jin Seok Lee,Kyu Yun Chai,Department of Bionanochemistry,College of Natural Science,Wonkwang University,Jeonbuk,Korea Hye-Jung Kim,Department of Family Medicine,Wonkwang University Hospital,Wonkwang University,Jeonbuk,Korea |
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| Abstract: | AIM: To investigate the anti-proliferative and apoptotic effects of Chaga mushroom (Inonotus obliquus) water extract on human hepatoma cell lines,HepG2 and Hep3B cells. METHODS: The cytotoxicity of Chaga extract was screened by 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay. Morphological observation,flow cytometry analysis,Western blot were employed to elucidate the cytotoxic mechanism of Chaga extract. RESULTS: HepG2 cells were more sensitive to Chaga extract than Hep3B cells,as demonstrated by markedly reduced cell viability. Chaga extract inhibited the cell growth in a dose-dependent manner,which was accompanied with G0/G1-phase arrest and apoptotic cell death. In addition,G0/G1 arrest in the cell cycle was closely associated with down-regulation of p53,pRb,p27,cyclins D1,D2,E,cyclin-dependent kinase (Cdk) 2,Cdk4,and Cdk6 expression. CONCLUSION: Chaga mushroom may provide a new therapeutic option,as a potential anticancer agent,in the treatment of hepatoma. |
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| Keywords: | Inonotus obliquus Cell cycle G0/G1 arrest Apoptosis |
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