三氯乙烯对接触工人外周血微核率的影响

目的　研究空气中三氯乙烯 (trichloroethylene ,TCE)浓度和接触工人外周血淋巴细胞微核发生率及尿中三氯乙酸 (trichloroacticacid ,TCA)浓度的剂量 反应关系 ,在染色体水平上探讨三氯乙烯对人体可能产生的危害以及接触 反应关系。方法　选择接触三氯乙烯至少三个月的工人和无接触三氯乙烯及其它有毒物质的某防疫站就业前体检工人作为研究对象 ,测定空气中三氯乙烯浓度、个体接触三氯乙烯浓度、外周血淋巴细胞微核发生率和尿中三氯乙烯的浓度。结果　接触组外周血淋巴细胞微核发生率 ,与对照组相比有升高 (P <0 0 1) ;个体接触三氯乙烯浓度和尿中三氯乙酸浓度呈正相关关系 (r =0 .761,P <0 0 0 1) ,而个体接触三氯乙烯浓度、尿中三氯乙酸浓度与外周血微核发生率均无相关关系。结论　①本现场研究表明三氯乙烯接触可导致微核生成增加 ;但不能确定三氯乙烯的遗传毒性作用 ;②尿中三氯乙酸浓度可以作为三氯乙烯接触水平的评价指标。

The effect of trichloroethylene on micronucleus formation in periphery lymphocytes of exposured workers

Objective To study the dose response correlation among the air concentration of trichloroethylene(TCE) in working place, the formation of micronucleus in periphery lymphocytes and the level of trichloroactic acid (TCA) in urine. Methods The study objects were selected from the workers having been exposed to TCE for at least three months, while those workers who had never been exposed to TCE or other toxicants served as control group. The air concentration of TCE and individual exposure level of TCE, the rate of micronucleus in periphery lymphocytes and the level of TCA in urine were measured. Results The formation of micronucleus in TCE exposure group was higher than that in control group(P<0.01). There was a positive correlation between individual TCE exposure level and the concentration of TCA in urine(r=0.761, P<0.001). However, there was no correlation among the individual TCE exposure level, the uric TCA concentration and the formation of micronucleus. Conclusion Trichloroethylene exposure could induce the formation of micronucleus in this field study. However, it seemed to be unable to evaluate the genetic toxicity of TCE in this case. The concentration of uric TCA could be used as biomaker of TCE exposure level.