| 分泌抗人A型、B型单克隆抗体细胞系的建立及初步应用 |
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| 引用本文： | 陈小龙,史良如,倪斌,周瑶玺. 分泌抗人A型、B型单克隆抗体细胞系的建立及初步应用[J]. 南京医科大学学报(自然科学版), 1988, 0(2) |
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| 作者姓名： | 陈小龙 史良如 倪斌 周瑶玺 |
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| 作者单位： | 南京医学院微生物学教研室(陈小龙)，武汉生物制品所免疫研究室(史良如)，微南京医学院生物学教研室(倪斌)，南京医学院微生物学教研室(周瑶玺) |
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| 摘 要： | 本文共得到分泌血型抗体的杂交瘤细胞6株,其中抗A 5株,抗B 1株。杂交瘤细胞系AN3/77、92、58、60、90(抗A)和AN1/4、25、45、40(抗B),经详细鉴定,其结果如下:(1)抗A 细胞系和抗B 细胞系在试管中传代3个月以上,稳定地分泌抗体,属鼠IgM。(2)小鼠腹腔接种后,腹水抗体效价为1:10000～1:20000(抗A)和1:320000～1:640000(抗B)。(3)吸附试验和阻断试验,证明抗A McAb 和抗B McAb 具有高度特异性。(4)随机抽样人血样品656份,用常规标准血清和本文所得两种单抗体,进行血型鉴定,结果二者完全相符。
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| 关 键 词： | 抗体 单克隆 ABO血型系统 人类 |
ESTABLISHMENT OF HYBRIDOMA CELL LINES SECRETING MONOCLONAL ANTIBODY AGAINST HUMAN RED CELLS(ANTI-A OR ANTI-B)AND PRELIMINARY APPLICATION IN BLOOD GROUPING |
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| Chen Xiaolong,et al.. ESTABLISHMENT OF HYBRIDOMA CELL LINES SECRETING MONOCLONAL ANTIBODY AGAINST HUMAN RED CELLS(ANTI-A OR ANTI-B)AND PRELIMINARY APPLICATION IN BLOOD GROUPING[J]. Acta Universitatis Medicinalis Nanjing, 1988, 0(2) |
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| Authors: | Chen Xiaolong et al. |
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| Abstract: | Six murine hybridoma cell lines,5 secreting anti-A and secreting anti-B,were obtained.Two hybridoma cell lines;AN3/77,92,58,60,90(anti-A)and AN 1/4,25,45,40(anti-B)were well characterized,the resultsshowed:(1)The anti-A cell line and the anti-B cell line propagated invitro more than 3 months and stably secreted antibodies in murine IgM na-ture.(2)After inoculation of hybridoma cells into mouse peritoneal cavi-ty,the agglutinating titers of ascitic fluids ranged 1:10000--1:20000(anti-A)and 1:320000--1:640000(anti-B)respectively.(3)Absorption testand blocking test further proved the specificity of the both McAbs. |
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| Keywords: | antibodies monoclonal ABO blood-group system human |
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