肝脏隔离灌注大剂量5—Fu肝脏的酶组织化学研究

应用大鼠肝脏隔离灌注技术，经门静脉灌注大剂剂量５－Ｆｕ观察肝脏酶学变化。结果ＩＬＰ平衡液组：ＩＬＰ５－Ｆｕ１５０ｍｇ／ｋｇ组，ＩＬＰ－５Ｆｕ２５０ｍｇ／ｋｇ组，术后琥珀酸脱氢酶，镁－三磷酸苷酶，５‘－核苷酸酶，胆碱酯酶活性下降，糖原反应减弱，乳酸脱氢酶和酸性磷酸酶活性增强。

HISTOCHEMICAL STUDY OF THE LIVER ON ISOLATED LIVER PERFUSION (ILP) WITH LARGE DOSE 5-FU

Histochemical changes of the liver were observed using ILP technique with large dose 5-Fu in rats. The results confirmed that the activities of SDH,Mg2+-AT-Pase,5-NT ChE and PAS were decreased and the activities of LDH and ACP increased in groups of ILP without 5-Fu and ILP with 5-Fu(150 and 250mg/kg)postoperatively. The enzymatic activities began to return on the third postoperative day. The enzymatic activities in group of ILP with 500mg/kg 5-Fu had more remarkable changes and did not return to normal on the third postoperative day,but they return to normal on the sev-enth postoperative day. The changes of enzymatic activities were in agreement with those of hepatic ultrastructure. The results demonstrated that the regional chemotherapy applying ILP within MTD of 5-Fu slightly affected the metabolic functions of liver. If 5-Fu doses should exceed MTD,the metabolic functions of hepatocytes were obviously in-jured