EB病毒蛋白对抗角蛋白自身抗体产生的影响

目的　研究EB病毒 (EBV)蛋白对B细胞产生抗角蛋白自身抗体 (AKautoAb)的影响。方法　用紫外线 (UV)灭活和热处理经EBV刺激培养的人脐带血B细胞 ,因ELISA法检测培养上清中AKautoAbIgG和IgM的水平。结果　UV灭活组IgG 18天以后各时间点有显著变化 (P <0 .0 5) ,IgM 2 6天与其它时间点有显著差异 (P <0 .0 5) ;热处理组IgG和IgM无明显变化 (P >0 .0 5)。结论　UV灭活EBV可诱导抗体的产生 ,而热处理EBV却不能 ,提示EBV蛋白成分可能是诱导抗体产生因素 ,这为深入研究影响AKautoAb产生的EBV功能蛋白奠定了基础

The Effect of Epstein-Barr Virus Protein on the Production of Antikeratin Autoantibodies

Objective To investigate the effect of Epstein -Barr virus(EBV) protein on the production of antikeratin autoantibody(AK au to Ab) by cultured human B cells.Methods The purified human umbilical cord blood B cells w ere treated respectively with UV and Heat-inactivated EBV and cultured in Vit ro.AK auto Ab IgG and IgM in the supernatant of culture fluid were detected by ELISA. Results In UV-inactivated EBV group,the AK auto Ab IgG p roduction (A value) of various time points after the 18 days increased signific antly(P<0.05),the A value of IgM just increased at the 26th day and had si gnificant difference compared with that of other time points(P<0.05).The A K auto Ab IgG and IgM A value in heat-inactivated EBV group didn’t increased w ith time(P>0.05).Conclusion The result indicates that EBV protein might be the effective factors in inducing the production of AK auto Ab, and this may be of great value in further researching the effects of functional EBV protein on the production of AK auto Ab.