| 钙纳米颗粒作为血吸虫病抗独特型抗体疫苗佐剂的研究 |
| |
| 引用本文： | 钟石根,冯振卿,仇镇宁,李玉华,王祝鸣,李芸茜,管晓虹.钙纳米颗粒作为血吸虫病抗独特型抗体疫苗佐剂的研究[J].中国血吸虫病防治杂志,2001,13(6):321-323. |
| |
| 作者姓名： | 钟石根 冯振卿 仇镇宁 李玉华 王祝鸣 李芸茜 管晓虹 |
| |
| 作者单位： | 南京医科大学医学分子生物学研究所,南京,210029 |
| |
| 基金项目： | 国家自然科学基金资助课题(30170836)及南京医科大学创新基金资助课题. |
| |
| 摘 要： | 目的 探索钙（Ca）纳米颗粒作为日本血吸虫病抗独特型抗体NP30疫苗佐剂的可行性。方法 将钙纳米颗粒与NP30制备成Ca-NP30结合物（Ca-NP30)，主动免疫BALB/c小鼠，观察其对小鼠的保护性作用并探讨其免疫保护机制。结果 Ca纳米颗粒可增强NP30对宿主的保护性作用，减虫率明显提高，从单用NP30的30.4%提高到57.8%；血清特异性抗体IgG水平较对照组显著升高；足垫试验可引发迟发型变态反应。结论 Ca纳米颗粒可作为血吸虫病抗独特型抗体NP30疫苗的佐剂，其作用机制与同时引起宿主体液免疫和细胞免疫应答增强有关。
|
| 关 键 词： | 钙 纳米颗粒 血吸虫病 抗独特型抗体 疫苗佐剂 |
| 文章编号： | 1005-6661(2001)06-0321-04 |
| 修稿时间： | 2001年9月18日 |
CALCIUM NANOPARTICLES AS VACCINE ADJUVANT OF ANTI-IDIOTYPIC ANTIBODY FORSCHISTOSOMIASIS |
| |
| ZHONG Shi-gen,FENG Zhen-qing,QIU Zhen-ning,LI Yu-hua,WANG Zhu-ming,LI Yun-qian,GUAN Xiao-hong Institute of Medical Molecular Biology,Nanjing Medical University,Nanjing.CALCIUM NANOPARTICLES AS VACCINE ADJUVANT OF ANTI-IDIOTYPIC ANTIBODY FORSCHISTOSOMIASIS[J].Chinese Journal of Schistosomiasis Control,2001,13(6):321-323. |
| |
| Authors: | ZHONG Shi-gen FENG Zhen-qing QIU Zhen-ning LI Yu-hua WANG Zhu-ming LI Yun-qian GUAN Xiao-hong Institute of Medical Molecular Biology Nanjing Medical University Nanjing |
| |
| Institution: | ZHONG Shi-gen,FENG Zhen-qing,QIU Zhen-ning,LI Yu-hua,WANG Zhu-ming,LI Yun-qian,GUAN Xiao-hong Institute of Medical Molecular Biology,Nanjing Medical University,Nanjing 210029 |
| |
| Abstract: | Objective To investigate whether calcium(Ca) nanoparticles can be used as vaccine adjuvant of anti-idiotypic antibody for schistosomiasis. Methods Ca nanoparticles-NP30 conjugate (Ca-NP30) was prepared, BALB/c mice were immunized with Ca-NP30, and effects on protective immunity was evaluated and its mechanisms of immune response was analyzed. Results Ca nanoparticles could enhance protective immunity of NP30 in mice, from 30 4%(NP30 alone) to 57 8%; vaccination with Ca-NP30 significantly increased production of IgG antibodies against NP30; footpad test could elicit delayed type hypersensitivity. Conclusions Ca nanoparticles could act as a vaccine adjuvant of anti-idiotypic antibody NP30 for schistosomiasis. Ca-NP30 could enhance humoral and cellular immune responses at the same time. |
| |
| Keywords: | Calcium Nanoparticles Schistosomiasis Anti-idiotypic antibody Vaccine adjuvant |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
