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| 生物衍生骨体外复合构建组织工程化骨修复兔桡骨-骨膜缺损:血管化进程及其生物力学性能 | |
| 引用本文： | 马雪峰,朱肖奇,贺用礼,郭浩,肖新跃.生物衍生骨体外复合构建组织工程化骨修复兔桡骨-骨膜缺损:血管化进程及其生物力学性能[J].中国组织工程研究与临床康复,2010,14(12). |
| 作者姓名： | 马雪峰 朱肖奇 贺用礼 郭浩 肖新跃 |
| 作者单位： | 1. 深圳平乐骨伤科医院创伤骨科,广东省深圳市,518000 2. 深圳市宝安区人民医院骨二科,广东省深圳市,518000 3. 石门县人民医院骨科,湖南省石门县,415300 4. 南华大学生物力教研室,湖南省衡阳市,421001 |
| 摘 要： | 背景:目前组织工程骨的支架材料,力学性能和体内血管化问题仍没有很好解决,因而制约了临床的广泛开展.目的:观察生物衍生骨体外复合骨髓间充质干细胞构建组织化工程骨修复兔桡骨一骨膜缺损的血管化进程和生物力学性能.方法:取新西兰大白兔的骨头制各生物衍生骨;体外分离和培养兔骨髓间充质干细胞,定向诱导骨髓间充质干细胞向成骨细胞分化,再将诱导的成骨细胞接种到生物衍生骨支架上制成组织工程骨.取大白兔25只,制成骨膜桡骨缺损模型,其中5只兔不做处理为空白组;另外20只兔左前肢为对照组单纯植入生物衍生骨,右前肢为实验组植入组织工程骨术后2,4,8,12周分别取5只兔标本,观察血管面积和缺损区植入骨的生物力学性能.结果与结论:术后4周实验组和对照组血液循环丰富,血管面积较大,12周血管面积趋于稳定,接近正常状态;2,4,8周的实验组和对照组血管面积与空白组比较差异有显著性意义(P<0.05).8周,与对照组比较,实验组修复区的血管网排列规律,12周形态结构已接近正常骨组织,力学性能明显增强.结果证实生物衍生骨复合骨髓间充质干细胞后有较好的血管化进程和力学性能,是修复骨缺损的一种较好的治疗方法. |
| 关 键 词： | 生物衍生骨 骨髓间充质干细胞 诱导 组织工程化骨 血管化 生物力学 |
Repair of radius-periosteum defects using in vitro constructed tissue engineered bone in rabbits:Vascularization and biomechanical property |
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| Ma Xue-feng,Zhu Xiao-qi,He Yong-li,Guo Hao,Xiao Xin-yue.Repair of radius-periosteum defects using in vitro constructed tissue engineered bone in rabbits:Vascularization and biomechanical property[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2010,14(12). | |
| Authors: | Ma Xue-feng Zhu Xiao-qi He Yong-li Guo Hao Xiao Xin-yue |
| Institution: | Ma Xue-feng1,Zhu Xiao-qi2,He Yong-li3,Guo Hao2,Xiao Xin-yue4 1Department of Orthopaedic Trauma,Shenzhen Pingle Orthopaedic Hospital,Shenzhen 518000,Guangdong Province,China,2Second Department of Orthopaedics,People\'s Hospital of Shenzhen Baoan Area,3Department of Orthopaedics,People\'s Hospital of Shimen County,Shimen 415300,Hunan Province,4Department of Biomechanics,University of South China,Hengyang 421001 |
| Abstract: | BACKGROUND:The biomechanical property and in vivo vascularization restrict the widely application of tissue engineering bone in clinic.OBJECTIVE:To study the vascularization and biomechanical property of tissue engineered bone in vitro constructed by bio-derived bone combined with bone marrow mesenchymal stem cells(BMSCs)in repair of radius-periosteum defects in rabbits.METHODS:The bio-derived bone was prepared using bones of New Zealand white rabbits,and the BMSCs of rabbits were harvested and cultured in ... |
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