IL-2基因转导CD3AK细胞免疫学功能的研究

目的 :观察白细胞介素 2 (IL 2 )基因转导后CD3AK细胞免疫学功能的变化。方法 :应用逆转录病毒载体将IL 2基因转导入CD3AK细胞。检测转导细胞中特异性NeoR 基因、培养上清IL 2的表达水平及转导CD3AK细胞的体外增殖活性、细胞毒活性和细胞表型。结果 :从转导细胞mRNA中扩增出长度为 347bp的特异性NeoR 基因片段 ,转导细胞培养上清的IL 2表达水平显著增高 ,体外增殖活性和细胞毒活性均强于未转导组细胞 ,CD4 /CD8 值升高。结论 :PLIL 2SN逆转录病毒转导CD3AK细胞后 ,IL 2基因得到表达并增强CD3AK细胞的免疫学功能

Research on Immunologic Functions of Interleukin-2 Gene Transducted CD3AK Cells by Retroviral Vector PLIL-2SN

Objective This experiment was designed to observe the immunologic functions of CD 3AK cells into which interleukin 2(IL 2) gene had been transducted.Methods The post transfer CD 3AK cells' special Neo R gene and cell immunologic functions including IL 2 expression,proliferation,cytotoxicity and cell phonetype were detected.Results The specific 347?bp Neo R gene was amplified in post transfer cells.The post transfer cells expressed higher IL 2, proliferation and cytotoxi~city ability. It was also found that the ratio of CD 4 T cell to CD 8 T cell increased in post transfer group. Conclusion Transducting IL 2 gene into CD 3AK cells could enhance their immunologic functions.