Contribution of the IdyllaTM System to Improving the Therapeutic Care of Patients with NSCLC through Early Screening of EGFR Mutations

Epidermal growth factor receptor (EGFR) genotyping, a critical examen for the treatment decisions of patients with non-small cell lung cancer (NSCLC), is commonly assayed by next-generation sequencing (NGS), but this global approach takes time. To determine whether rapid EGFR genotyping tests by the Idylla^TM system guides earlier therapy decisions, EGFR mutations were assayed by both the Idylla^TM system and NGS in 223 patients with NSCLC in a bicentric prospective study. Idylla^TM demonstrated agreement with the NGS method in 187/194 cases (96.4%) and recovered 20 of the 26 (77%) EGFR mutations detected using NGS. Regarding the seven missed EGFR mutations, five were not detected by the Idylla^TM system, one was assayed in a sample with insufficient tumoral cells, and the last was in a sample not validated by the Idylla^TM system (a bone metastasis). Idylla^TM did not detect any false positives. The average time between EGFR genotyping results from Idylla^TM and the NGS method was 9.2 ± 2.2 working days (wd) (12.6 ± 4.0 calendar days (cd)). Subsequently, based on the Idylla^TM method, the timeframe from tumor sampling to the initiation of EGFR-TKI was 7.7 ± 1.2 wd (11.4 ± 3.1 cd), while it was 20.3 ± 6.7 wd (27.2 ± 8.3 cd) with the NGS method (p < 0.001). We thus demonstrated here that the Idylla^TM system contributes to improving the therapeutic care of patients with NSCLC by the early screening of EGFR mutations.