肌肉及骨内植入磷酸钙骨水泥/骨形态发生蛋白成骨分析

目的成骨能力和降解性能是评价骨移植材料的重要指标,通过研究磷酸钙骨水泥(calciumphosphatecement,CPC)/骨形态发生蛋白(bonemorphogeneticprotein,BMP)(CPC/BMP)复合人工骨在肌肉和骨缺损处的成骨性能和降解规律,探讨其临床应用价值。方法研制CPC/BMP复合物,植入小鼠肌袋和兔桡骨15mm骨缺损,以大体观察、扫描电镜和能谱分析的方法研究材料周围新骨形成的特点和CPC/BMP变化规律。结果CPC植入小鼠肌袋内不能诱导成骨,CPC/BMP植入后有新骨生成,同时材料出现降解。植入骨缺损后,CPC/BMP复合物和单纯的CPC均可以促进新骨形成,但后者骨修复能力弱,材料降解速度缓慢。CPC/BMP植入4周有新骨形成,材料形态出现变化,晶体结构被逐渐破坏。24周有板层骨长入并与材料紧密结合,骨缺损初步修复,材料被溶解成规则的蜂窝状框架结构。X-射线能谱分析结果表明,随着时间的延长,新骨钙化程度逐渐增高,24周时已经与正常骨接近。结论CPC/BMP生物活性人工骨降解性能理想,对骨缺损有较强的修复能力,可望成为新型的骨缺损修复材料。

Osteogenesis analysis of calcium phosphate cement /bone morphog enetic protein implanted into muscle or bone

Aim To study the degradation rate,osteo genesis character and bone-b on ding capability of calc ium phosphate cement (CPC)combined with bone morphogen etic protein(BMP )implanted into bone or muscle and to investigate the clinica l values o f the compound artificial bone.Methods A new type of non-ceramic h ydroxyapatite cement of CPC was synthesized,and CPC /BMP composite was implant ed into muscle and radius of mice for repairing 15mm bone defect to study i ts biodegrade rate,osteogenesis character and bone-bonding capabil ity.Samples were observed by scanni ng electron microscope(SEM)and energy dispersion analy sis and X -ray(EDAX).Results Compared with CPC,the CPC /BMP composite had the ideal degradation rate and osteogen esis character.When implanting the comp osite into muscle pouches of mic e,new bone formation was observed with signifi cant degradation of the material.No new bone formation and degradation of the m aterial were fou nd in CPC group.Implanting the CPC /BMP into 15mm-defect i n the middle of the radial diaphysis of mice,new bone formed at 4weeks with t he morphologic change of the material and the damage of crystal structure,and t hen new bone had bridged the gap between the proximal and distal fragments at th e end of 24weeks,and most of the CPC /BMP composite was degraded and a bsorb ed.Only little new bone formed in the CPC group and no visible degradation was found.EDAX in-dicated calcification was signific antly higher in the CPC /BMP group than that of CPC group,and the degree of calcification in the CPC /BMP group was close to that of the normal bone.Conclusion CPC /BMP composite has t he ideal degradation rate with the g reat potential in bone defect repair,and i t is expected to use as grafting mate rial in dealing with segmental bone d e-f ect in clinic.