增殖性玻璃体视网膜病变的药物体外筛选试验

目的：研究４种药物对体外成纤维细胞培养的增殖抑制作用及有效浓度。方法：对传代培养的成纤维细胞经一定浓度的柔红霉素、阿霉素、地塞米松、５－ＦＵ培养４８ｈ后，观察细胞生长情况，并分别求得对兔皮肤成纤维细胞的半数抑制浓度（ＩＤ５０）。结果：柔红霉素、阿霉素对体外成纤维细胞的增殖均有显著抑制作用，呈浓度依赖性改变；地塞米松和５－ＦＵ的抑制作用相对较弱，但地塞米松分别与其他３种药物的半数抑制浓度合用后的效果，比单用上述４种药物的抑制作用明显增强。结论：柔红霉素、阿霉素能有效抑制体外兔成纤维细胞，并提示与地塞米松合用可能成为有价值的防治增殖性玻璃体视网膜病变（ＰＶＲ）的用药方式

Selection of Therapeutic Agents for Proliferative Vitroretinopathy in Vitro

Objective:To investigate the inhibitory activity and effective concentration of four drugs against the proliferation of fibroblasts of rabbit dermal in vitro .Method:Cultrred fibroblasts were exposed for 48 hours to a different concentration of daunomycin,doxorubicin,5-Fluorouracil and dexamethason solution.The suppresion of the proliferation of fibroblasts was investigated and the median lethal dose(ID 50 ) was caculated respectively.Results:Daunomycin and doxorubicin significantly suppressed the prolifreation of fibroblasts in vitro ,showing dose-dependent manner,dexamethason and 5-Fluorouracil demonstrated an inhibiting effect not as strong as the other two drugs.The inhibiting effect of combination of dexamexason with other three drugs was also evaluated,and it was found to be much better than in single use.Conclusion:Exposure of daunomycin and doxorubicin at certain concentration can effectively inhibit the proliferation of rabbit dermal fibroblast in vitro .Combination of dexamethason with other antimetabolites was thought to be of significant value possibly on treatment of PVR.