长期心理应激对大鼠血浆皮质酮、儿茶酚胺水平及免疫功能的影响

①目的研究长期心理应激对大鼠血浆皮质酮、儿茶酚胺水平以及免疫功能的影响并探讨其机制。②方法将40只成年大鼠随机分为3组：心理应激组、普通应激组和正常对照组，对实验组动物施以8周的长期应激刺激，检测各组大鼠血浆皮质酮、肾上腺素和去甲肾上腺素水平以及大鼠胸腺指数、脾脏指数和体外脾淋巴细胞转化率。③结果心理应激组大鼠血浆皮质酮水平显著低于普通应激组与正常对照组，肾上腺素、去甲肾上腺素水平显著高于普通应激组和正常对照组，而胸腺指数、脾脏指数及脾淋巴细胞。H—TdR掺人量均显著低于普通应激组和正常对照组（F=4．13～8．66，q=3．02～5．42，P〈0．01、0．05）。④结论长期心理应激可显著降低大鼠的免疫功能。

EFFECT OF LONG TERM PSYCHOLOGICAL STRESS ON PLASMA CORTICOSTERONE, CATECHOLAMINE LEVEL AND IMMUNOLOGICAL FUNCTION IN RATS

Objective To study the effect of long term psychological stress on plasma corticosterone, catecholamine level and immunological function in rats. Methods Forty rats were divided into three groups: psychological stress(PS) group, common stress(CS) group and normal control(NC) group. The blood samples, the thymus and the spleen were taken after completion of eight-week's stress. Corticosterone and catecholamine level and T cell proliferation were assayed, and the thymus and spleen index calculated after weighing. Results Compared with the animals in CS and NC group, the corticosterone level of the PS group decreased significantly, and the index of thymus and spleen, the T cell proliferation also decreased significantly in PS group (F=(4.13-)8.66;q=3.02-5.42;P<0.01,0.05). Conclusion The immunological function in rats is markedly suppressed after long term psychological stress.