转染CTGF基因对乳腺癌细胞MMP－2及TIMP－2表达的影响

目的: 通过研究结缔组织生长因子（CTGF）基因对人乳腺癌MCF-7细胞株基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶组织抑制因子-2（TIMP-2）表达的影响，深入探讨CTGF在乳腺癌侵袭和转移中的作用机制。方法: 采用脂质体介导法，将CTGF基因瞬时转染体外培养的人MCF-7细胞，并用免疫荧光、RT-PCR和蛋白质印迹法检测其转染成功与否;再采用蛋白质印迹法检测转基因MCF-7 MMP-2及TIMP-2表达改变。结果: 与对照组相比，转染CTGF基因的MCF-7，其CTGF mRNA和蛋白表达均显著增高，MMP-2蛋白表达水平显著增高，而TIMP-2蛋白表达水平显著降低。 结论: CTGF可能通过增加乳腺癌细胞MMP-2表达及抑制TIMP-2的生成而起到促进乳腺癌侵袭和转移的作用。

Effect of gene CTGF transfection on the expression of MMP-2 and TIMP-2 in human breast cancer cells

Objective: To investigate the effect of connective tissue growth factor(CTGF) on the expression of matrix metalloproteinase-2(MMP-2) and tissue inhibitor of metalloproteinase-2(TIMP-2) in human breast cancer line MCF-7.This study was designed to explore the underlying mechanism for the role of CTGF in the development of breast cancer.Methods: Lipofectin method was used to transfect CTGF vectors into MCF-7;immunofluorescence,RT-PCR and Western blot analysis were used to detect their transfection efficiency.The expressions of MMP-2 and TIMP-2 were determined by Western blot analysis.Results: Immunofluorescence,RT-PCR and Western blot analysis showed a successful transfection of CTGF vectors into MCF-7.MCF-7 transfected with CTGF gene showed an increased expression of MMP-2 protein significantly,compared with control groups.On the other hand,MCF-7 transfected with CTGF gene showed a decreased expression of TIMP-2 protein significantly,compared with control groups.Conclusion: It is possible that CTGF could promote the development of breast cancer by effectively enhancing the expressions of MMP-2 and TIMP-2 in MCF-7 cells.