玻璃苣醇提物对慢性抑郁模型小鼠脑组织中神经递质的影响

目的:通过建立慢性应激小鼠抑郁模型,观察玻璃苣对慢性应激抑郁模型小鼠脑内5-羟色胺、去甲肾上腺素(NE)、多巴胺3种单胺类神经递质的影响,初步探讨玻璃苣对抑郁模型小鼠的影响及其作用机制。方法:利用孤养和长期不可预见性温和应激(CUMS)建立慢性应激小鼠抑郁模型,采用酶联免疫吸附测定法(ELISA)测定慢性应激抑郁模型小鼠脑内单胺类神经递质的变化。结果:模型组小鼠脑内5-羟色胺、NE、多巴胺含量明显低于正常组;与模型组相比,玻璃苣醇提物高、中剂量组小鼠脑内5-羟色胺、NE、多巴胺的含量均有升高,且差异有统计学意义。玻璃苣醇提物低剂量组小鼠脑内5-羟色胺、NE、多巴胺的含量也有升高趋势,但差异无统计学意义。结论:玻璃苣能够提高慢性应激抑郁模型小鼠脑内单胺类神经递质(5-羟色胺、NE、多巴胺)的含量,玻璃苣对小鼠脑内神经递质的作用可能是其对抑郁模型小鼠产生影响的可能机制。

Effects of the ethanol extractive of Borago officinalis on neurotransmitter in the brain tissue of mouse model of chronic depression

Objective: The effects of the extractive of Borago officinalis on norepinephrine(NE),dopamine and 5-hydroxytryptamine(5-HT)in mouse model of depression with chronic stress were investigated after establishing of chronic stress mouse models of depression;and to discuss its prevention and cure effects and their possible mechanisms.Methods: Established chronic stress mouse models of depression by using singly housed and long-trem unpredictable mild stress(CUMS);and to determine the change of brain monoamine neurotransmitters of chronic stress depression model mice by using enzyme-linked immunosorbent assay(ELISA).Results: The monoamine neurotransmitters(5-HT,NE,dopamine)contents in model mice were significantly less than normal group.The high,medium group of ethanol extractive from Borago officinalis all could increase the contents of 5-HT,NE,dopamine of mice with chronic stress depression;and the low group of ethanol extractive from Borago officinalis approached a raising trend,but no statistical significance.Conclusion: The Borago officinalis can elevate the contents of the 5-HT,NE and dopamine in the brian which were the possible mechanisms of the prevention and cure effect of the Borago officinalis.