百草枯一次灌胃构建家兔肺间质纤维化模型

目的： 构建百草枯致肺间质纤维化动物模型。方法： 25只家兔随机分为实验组（20只）和对照组（5只）。实验组家兔以40 mg/kg体质量百草枯一次性灌胃，并于染毒后第3、7、14、28天处死，对照组生理盐水灌胃并于第28天处死。观察两组家兔的一般情况、肺组织大体结构和肺组织病理学改变。结果： 实验组家兔在染毒后1 h即出现中毒性改变，肺组织在第28天出现明显的纤维化改变。结论：百草枯灌胃法能可靠地构建出肺纤维化动物模型。

Establishment of a rabbit model of pulmonary fibrosis induced by stomach lavaging of paraquat

Objective: To develop a method of establishing rabbit model of paraquat-induced pulmonary fibrosis.Methods: Twenty-five rabbits were divided randomly into the experimental group(n=20) and control group(n=5).Paraquat(40 mg/kg) was administrated to the animals of the experimental group by stomach lavaging once of paraquat.The rabbits were killed on the day 3,7,14 and 28 after paraquat administration.The rabbits in the control group were treated by stomach lavaging once of physiological saline and killed on the 28th day.The general conditions,macroscopic structure and pathological changes of lung in the rabbits of both groups were examined and analyzed.Results: The manifestation of intoxication was shown at 1 h after paraquat administration,and obvious fibrosis of the lung tissue was observed on the day 28 in the experimental group.Conclusion:A rabbit model of pulmonary fibrosis can be established reliably by stomach lavaging of paraquat.