吗啡对乳腺癌MCF-7细胞生存素及livin表达的影响

目的:探讨吗啡对人乳腺癌MCF-7细胞生存素(survivin)和livin的表达及细胞增殖与凋亡的影响。方法:人乳腺癌MCF-7细胞以1×103个/ml密度接种在6孔板内,随机分为对照组,0.1μmol/L吗啡组,1.0μmol/L吗啡组。于吗啡孵育24 h时,分别采用RT-PCR法和蛋白质印迹法测定细胞内生存素及livin mRNA及蛋白表达,流式细胞术检测细胞凋亡改变;于吗啡孵育24,48及72 h时采用MTT法检测细胞增殖改变情况。结果:与对照组比较,0.1μmol/L吗啡组及1.0μmol/L吗啡组生存素和livin蛋白及mRNA表达水平均显著降低(P<0.01),0.1μmol/L吗啡组及1.0μmol/L吗啡组细胞增殖降低而细胞凋亡增加(P<0.01)。结论:吗啡通过下调MCF-7细胞生存素和livin的表达,从而抑制细胞增殖并促进细胞凋亡。

Effect of morphine on the expression of survivin and livin in human breast cancer cells

Objective: To investigate the effect of morphine on proliferation and apoptosis in human breast cancer cell line MCF-7,with a focus on the expression of survivin and livin.MCF-7 cells were seeded in 6-well plates(1×103/ml) and divided into 3 groups: group Ⅰ normal control(group C),group Ⅱ was exposed to 0.1 μmol/L morphine(group M1),group Ⅲ was exposed to 1.0 μmol/L morphine(group M2).The expression of survivin and livin in different group of cells was detected by RT-PCR and Western blot at 24 hours of incubation with morphine.The apoptosis of cells was determined by flow cytometry at 24 hours of incubation with morphine.The proliferation of cells was determined by Thiazolyl blue tetrazolium bromide(MTT) method at 24,48,and 72 hours of incubation with morphine respectively.Results: Compared with group C,the expression of survivin and livin,and the proliferation level of group M1 and M2 was decreased significantly(P<0.01),however,the apoptosis rate of cells was increased significantly(P<0.01).Conclusion: Morphine could inhibit the proliferation of the cells and accelerate the cell apoptosis through down-regulating the expression of survivin and livin genes in human breast cancer cell line MCF-7.