OsmY缺失对伤寒沙门菌在高渗应激早期基因表达的影响

目的:研究伤寒沙门菌OsmY在高渗应激早期对其他基因表达的调节。方法:通过同源重组的方法利用自杀质粒制备伤寒沙门菌osmY基因缺陷变异株;采用伤寒沙门菌全基因组芯片比较野生株和osmY基因缺陷变异株在高渗应激早期的基因表达差异,并对其中部分表达差异基因进行实时定量PCR验证。结果:成功制备伤寒沙门菌osmY基因缺陷变异株;基因芯片结果显示,在高渗应激早期,与野生株相比,伤寒沙门菌osmY基因缺陷变异株有128个基因表达下调,有27个基因表达上调。实时定量PCR与芯片结果一致。结论:OsmY可作为一调节因子在伤寒沙门菌高渗应激早期对基因表达起重要调节作用。

Effect of OsmY on gene expressional regulation of Salmonella enterica serovar Typhi at early stage of hyperosmotic stress

Objective: To explore the effect of OsmY on gene expressional regulation of Salmonella entericaserovar Typhi(S.Typhi) at the early stage of hyperosmotic stress.Methods: The osmY gene deleted mutant of S.Typhi was generated through homologous recombination mediated by a suicide plasmid;the gene expression profiles of the wild type strain and the osmY deleted mutant at early stage of hyperosmotic stress was investigated by genomic microarray assay;qRT-PCR was performed to validate the results of microarray assay.Results: The osmY deleted mutant of S.Typhi was prepared successfully;analysis of genomic microarray assay showed that 27 genes were up regulated and 128 genes were down regulated in the osmY deleted mutant at the early stage of hyperosmotic stress compared to the wild type strain.The results of qRT-PCR assay were consistent with the results of microarray assay.Conclusion: osmY of S.Typhi may be a regulator played an important role in regulating gene expression at early stage of hyperosmotic stress.