| 子宫内膜腺癌细胞系的凝集素受体表达 |
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| 引用本文： | 刘祖林,刘金伟,陈代雄,吴中明.子宫内膜腺癌细胞系的凝集素受体表达[J].贵州医药,2001,25(1):9-10. |
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| 作者姓名： | 刘祖林 刘金伟 陈代雄 吴中明 |
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| 作者单位： | 1. 遵义医学院分子生物学研究室
2. 遵义医学院微生物学研究室 |
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| 摘 要： | 目的：观察子宫内膜腺癌细胞系（JEC）凝集素受体的表达。方法：用ABC免疫组化技术检测JEC瘤源细胞第72代、第275代及裸鼠移植瘤细胞的WGA、ConA、PASA、PNA、PHA、DBA、LCA、SBA、UEA、BSL、RCA、JSJA等12种凝集受体。结果JEC的不同代次及鼠移植瘤细胞的6种凝集素（WGA、Cona、PSA、PHA、PCA、LCA）受体呈阳性反应，而对PNA、DBA、SBA、UEA、BSL和SJA则呈阴性。结论：JEC12种凝集素受体的显色反应是稳定的，并不因培养、冻存、传代及异种移植等因素影响而发生改变。
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| 关 键 词： | 凝聚互受体 子宫内膜腺癌细胞素 免疫组织化学 子宫内膜癌 |
Expression of Lectins receptors of JEC for a endometrial ad cell line |
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| liu Zulin et al.Expression of Lectins receptors of JEC for a endometrial ad cell line[J].Guizhou Medical Journal,2001,25(1):9-10. |
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| Authors: | liu Zulin |
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| Institution: | liu Zulin et al.Department of Molecular Biology,Zunyi Medical College,Zunyi,563003 |
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| Abstract: | Objective To observe the expression of lectins in JEC, a endometrial adenocarcinoma cells line. Methods 12 lectins (WGA,ConA,PSA,PNA,PHA,DBA,LCA,SBA,UEA,BSL,RCA and SJA) receptors in cells smemars of original tumor ascites of 72th, 275th generation and nude mice transmissive tumor cells of JEC were studied by immunohistochemical ABC technique.Results WGA,ConA,PSA,PHA,RCA,LCA lectins receptors between different generations and nude mice transmissive tumor cells were positive and others were negative. Conclusion These lectins receptors stable in establishment process of JEC,they cannot be changed in effect at culture of freezing,handed down and heteroplastic transplantation. |
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| Keywords: | Lectins receptor Endometrial aderocinoma cell line Immunohisto chemical |
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