异体软骨痂移植的初步结果

目的 通过观察异体软骨痂移植后的生物学过程判断其作为植骨材料的可行性。方法 将1只SD大鼠的双侧股骨干造成闭合骨折，1周时切开获取软骨痂，－196℃冻存2周后移植于5只SD大鼠的左侧胫骨干部分缺损区（此骨缺损模型的成骨活动只表现为膜内化骨的方式），右侧植入异体松质骨作为对照组。将取材标本制成不脱钙切片，经亮绿和藏红T染色。结果 术后1周取材1例，缺损区实验侧和对照侧均未见有软骨组织和骨组织形成。术后2周处死其余4只大鼠，实验侧（3／4）可见有软骨组织和骨组织形成。骨组织内已有髓腔形成，骨组织周围是软骨组织，与宿主骨之间有纤维组织相隔。结论 异体软骨痂移植后未被吸收，可经软骨内化骨的方式产生骨组织，为软骨痂作为植骨材料的进一步研究和开发提供了初步依据。

THE PRELIMINARY RESULT OF OSTEOID CALLUS ALLOGRAFT FOR BONE HEALING

OBJECTIVE: To investigate the feasibility of osteoid callus allograft as a kind of bone healing promoting materials. METHODS: The osteoid callus was obtained at one week after bilateral femoral fracture of a SD rat, then was kept at -196 C for 2 weeks. The bone defect model which bone repair was in intra-membranous osteogenesis was made at bilateral tibial shaft in 5 rats, and filled with the osteoid callus in the left defect area, the right side was filled with allogenous cancellous as control group. The specimen were processed with undecalcified technique and the sections were staining with light blue and sofranin T. RESULTS: After 2 weeks, there were cartilage and bone formation in the defect area of osteoid callus graft group(3/4), medullary cavity formation in bone tissue with cartilage arround it, fibrous tissues between new bone and host bone. While there were no cartilage or bone formation in the control group. CONCLUSION: The allograft osteoid callus is not absorbed by immunological rejection, but changed into bone tissue through endochondral osteogenesis. It is inspiring to develop osteoid callus allograft as a kind of material for bone healing.