和厚朴酚抑制视网膜新生血管形成的实验研究

目的:观察和厚朴酚对视网膜新生血管形成的抑制作用。方法:氧诱导幼鼠建立视网膜NV模型。治疗组分别腹腔注射和厚朴酚100 mg/kg和50 mg/kg,每日2次共5 d;对照组注射等量BSS。视网膜铺片了解血管改变;组织切片计数突破视网膜内界膜血管内皮细胞核;免疫组化检测视网膜VEGF和MMP-2表达。结果:治疗组视网膜血管密度降低,新生血管数减少。治疗组突破内界膜胞核数较对照组明显减少;大剂量组减少更显著。治疗组视网膜VEGF和MMP-2表达较对照组明显减少;大剂量组更少,两者差异显著。结论:和厚朴酚可以有效抑制视网膜新生血管形成,呈剂量依赖关系。

Inhibitory effects of honokoil on retinal neovascularization in mice

AIM: To explore the inhibitory effect of honokiol on retinal NV. METHODS: Hyperoxia was induced in NV model in 60 mice.Twenty mice received an intraperitoneal injections of 100 mg/kg honokoil twice daily for 5 days as large-dosage group and 20 mice received 50 mg/kg as small one while 20 mice received BSS as control.The collagen IV immunohistochemical staining was used to observe retinal vessels.The effect was evaluated by counting the nuclei extending from retina to vitreous and expressions of VEGF and MMP-2. RESULTS: The reduced density of vessels in the treatment group were found in retinal.The number of nuclei was less in the treated groups,which in the lagre-dosage group was significantly less.The expressions to VEGF and MMP-2 were less in the treatment than in the control respectively.That in the large-dosage group was significantly less. CONCLUSION: Intraperitoneal injections of honokoil can effectively inhibit retinal NV,depending on dosage.