| 正常人骨髓粒单祖细胞的极限稀释微孔培养研究 |
| |
| 引用本文： | 黄士昂,王辨明,李崇渔,喻东姣. 正常人骨髓粒单祖细胞的极限稀释微孔培养研究[J]. 华中科技大学学报(医学版), 1987, 0(4) |
| |
| 作者姓名： | 黄士昂 王辨明 李崇渔 喻东姣 |
| |
| 作者单位： | 同济医科大学附属协和医院血液病研究室(黄士昂，王辨明，李崇渔)，同济医科大学附属协和医院血液病研究室(喻东姣) |
| |
| 摘 要： | 本文应用先进的极限稀释微孔培养技术,成功地培养出人骨髓粒-单祖细胞(CFU-GM),并在方法学上作了改进,能够获得完整清晰的集落形态。采用线性回归分析、标记性染色体、3_H-TdR参入、扫描电镜等多种手段证实了CFU-GM的克隆性生长和极限稀释微孔培养CFU-GM的优点。
|
| 关 键 词： | 极限稀释法 集落形成单位测定 粒-单祖细胞 正常人 |
The Research of Normal Human Bone Marrow CFU-GM by Limiting Dilution Microculture |
| |
| Huang Shiang,Wang Bianming,Li Chongyu,Yu Dongjiao Hematology Research Institute,Xiehe Hospital,Tongji Medical University,Wuhan. The Research of Normal Human Bone Marrow CFU-GM by Limiting Dilution Microculture[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 1987, 0(4) |
| |
| Authors: | Huang Shiang Wang Bianming Li Chongyu Yu Dongjiao Hematology Research Institute Xiehe Hospital Tongji Medical University Wuhan |
| |
| Abstract: | By using of the newly developed limiting diluting microculture we have succeeded in cultivating CFU-GM of humon bone marrow. The clones, developed by this method, are more concise, well identified and the cells are well separated in comparison with semiliquid agarculture. This method has further proved as an accurate one by using of linear regression analysis, H-TdR, and scanning elec tromicroscopy. |
| |
| Keywords: | limiting dilution colony-forming units assay CFU -GM normal adult |
| 本文献已被 CNKI 等数据库收录！ |
|
